Detail publikačního výsledku

Rapid and Ultrasensitive Method for Determination of Phytochelatin2 using High Performance Liquid Chromatography with Electrochemical Detection

ZÍTKA, O.; ŠKUTKOVÁ, H.; KRYŠTOFOVÁ, O.; ŠOBROVÁ, P.; ADAM, V.; ZEHNÁLEK, J.; BEKLOVÁ, M.; HUBÁLEK, J.; PROVAZNÍK, I.; KIZEK, R.

Originální název

Rapid and Ultrasensitive Method for Determination of Phytochelatin2 using High Performance Liquid Chromatography with Electrochemical Detection

Anglický název

Rapid and Ultrasensitive Method for Determination of Phytochelatin2 using High Performance Liquid Chromatography with Electrochemical Detection

Druh

Článek WoS

Originální abstrakt

Plants respond to heavy metal toxicity in a variety of different ways including synthesis of phytochelatins. The synthesis of phytochelatins is catalyzed by gama-Glu-Cys dipeptidyl transpeptidase named as phytochelatin synthase (PCS). The main aim of this study was to optimize high performance liquid chromatography coupled with electrochemical detector for determination of phytochelatin2. The optimized procedure was subsequently used for determination of the mentioned molecules with special attention aimed at the possibility to determine PC2 after activation of PCS in the tobacco BY-2 cells treated with different concentrations of cadmium(II) ions.

Anglický abstrakt

Plants respond to heavy metal toxicity in a variety of different ways including synthesis of phytochelatins. The synthesis of phytochelatins is catalyzed by gama-Glu-Cys dipeptidyl transpeptidase named as phytochelatin synthase (PCS). The main aim of this study was to optimize high performance liquid chromatography coupled with electrochemical detector for determination of phytochelatin2. The optimized procedure was subsequently used for determination of the mentioned molecules with special attention aimed at the possibility to determine PC2 after activation of PCS in the tobacco BY-2 cells treated with different concentrations of cadmium(II) ions.

Klíčová slova

cadmium, glutathione, phytochelatin, high performance liquid chromatography, electrochemical detector, tobacco BY-2 cell suspension

Klíčová slova v angličtině

cadmium, glutathione, phytochelatin, high performance liquid chromatography, electrochemical detector, tobacco BY-2 cell suspension

Autoři

ZÍTKA, O.; ŠKUTKOVÁ, H.; KRYŠTOFOVÁ, O.; ŠOBROVÁ, P.; ADAM, V.; ZEHNÁLEK, J.; BEKLOVÁ, M.; HUBÁLEK, J.; PROVAZNÍK, I.; KIZEK, R.

Rok RIV

2012

Vydáno

01.05.2011

ISSN

1452-3981

Periodikum

International Journal of Electrochemical Science

Svazek

2011 (6)

Číslo

5

Stát

Srbská republika

Strany od

1367

Strany do

1381

Strany počet

15

BibTex

@article{BUT50164,
  author="Ondřej {Zítka} and Helena {Vítková} and Olga {Kryštofová} and Pavlína {Adam} and Vojtěch {Adam} and Josef {Zehnálek} and Miroslava {Beklová} and Jaromír {Hubálek} and Valentýna {Provazník} and René {Kizek}",
  title="Rapid and Ultrasensitive Method for Determination of Phytochelatin2 using High Performance Liquid Chromatography with Electrochemical Detection",
  journal="International Journal of Electrochemical Science",
  year="2011",
  volume="2011 (6)",
  number="5",
  pages="1367--1381",
  issn="1452-3981"
}