Detail publikačního výsledku

Spectrometric and Voltammetric Analysis of Urease: Nickel Nanoelectrode as an Electrochemical Sensor

HUBÁLEK, J.; HRADECKÝ, J.; ADAM, V.; KRYŠTOFOVÁ, O.; HÚSKA, D.; MASAŘÍK, M.; TRNKOVÁ, L.; HORNA, A.; KLOSOVÁ, K.; ADÁMEK, M.; ZEHNÁLEK, J.; KIZEK, R.

Originální název

Spectrometric and Voltammetric Analysis of Urease: Nickel Nanoelectrode as an Electrochemical Sensor

Anglický název

Spectrometric and Voltammetric Analysis of Urease: Nickel Nanoelectrode as an Electrochemical Sensor

Druh

Článek recenzovaný mimo WoS a Scopus

Originální abstrakt

Urease is the enzyme catalyzing the hydrolysis of urea into carbon dioxide and ammonia. This enzyme is substrate-specific, which means that the enzyme catalyzes the hydrolysis of urea only. This feature is a basic diagnostic criterion used in the determination of many bacteria species. Most of the methods utilized for detection of urease are based on analysis of its enzyme activity (the hydrolysis of urea). The aim of this work was to detect urease indirectly by spectrometric method and directly by voltammetric methods. As spectrometric method we used is called indophenol assay.

Anglický abstrakt

Urease is the enzyme catalyzing the hydrolysis of urea into carbon dioxide and ammonia. This enzyme is substrate-specific, which means that the enzyme catalyzes the hydrolysis of urea only. This feature is a basic diagnostic criterion used in the determination of many bacteria species. Most of the methods utilized for detection of urease are based on analysis of its enzyme activity (the hydrolysis of urea). The aim of this work was to detect urease indirectly by spectrometric method and directly by voltammetric methods. As spectrometric method we used is called indophenol assay.

Klíčová slova

urease, electrochemical methods, nanotechnology, nanotube, nickel electrode, hanging mercury drop electrode, spectrometry

Klíčová slova v angličtině

urease, electrochemical methods, nanotechnology, nanotube, nickel electrode, hanging mercury drop electrode, spectrometry

Autoři

HUBÁLEK, J.; HRADECKÝ, J.; ADAM, V.; KRYŠTOFOVÁ, O.; HÚSKA, D.; MASAŘÍK, M.; TRNKOVÁ, L.; HORNA, A.; KLOSOVÁ, K.; ADÁMEK, M.; ZEHNÁLEK, J.; KIZEK, R.

Vydáno

16.07.2007

Nakladatel

MDPI

ISSN

1424-8220

Periodikum

SENSORS

Svazek

7

Číslo

1

Stát

Švýcarská konfederace

Strany od

1238

Strany do

1255

Strany počet

18

URL

http://www.mdpi.com/1424-8220/7/7/1238

Plný text v Digitální knihovně

http://hdl.handle.net/11012/68386

BibTex

@article{BUT44511,
  author="HUBÁLEK, J. and HRADECKÝ, J. and ADAM, V. and KRYŠTOFOVÁ, O. and HÚSKA, D. and MASAŘÍK, M. and TRNKOVÁ, L. and HORNA, A. and KLOSOVÁ, K. and ADÁMEK, M. and ZEHNÁLEK, J. and KIZEK, R.",
  title="Spectrometric and Voltammetric Analysis of Urease: Nickel Nanoelectrode as an Electrochemical Sensor",
  journal="SENSORS",
  year="2007",
  volume="7",
  number="1",
  pages="1238--1255",
  doi="10.3390/s7071238",
  url="http://www.mdpi.com/1424-8220/7/7/1238"
}