Detail publikačního výsledku

Electrophysiological effects of Voltage-Sensitive Dye di-4-ANEPPS in Rabbit and Guinea Pig Isolated Heart

FIALOVÁ, K.; KOLÁŘOVÁ, J.; SEKORA, J.; TANNENBERG, M.; NOVÁKOVÁ, M.; PROVAZNÍK, I.

Originální název

Electrophysiological effects of Voltage-Sensitive Dye di-4-ANEPPS in Rabbit and Guinea Pig Isolated Heart

Anglický název

Electrophysiological effects of Voltage-Sensitive Dye di-4-ANEPPS in Rabbit and Guinea Pig Isolated Heart

Druh

Stať ve sborníku v databázi WoS či Scopus

Originální abstrakt

Voltage-sensitive dye (VSD) di-4-ANEPPS is currently used in our laboratory for recording of monophasic action potentials (MAPs) in isolated hearts. In this study, its electrophysiological effects on rabbit and guinea pig myocardium were compared during staining with VSD and washout from it. Electrograms were recorded and heart rate changes and the types and frequency of arrhythmias were evaluated. Althought certain electrophysiological changes were present in the hearts of both species, these changes are mostly reversible, especially in rabbit hearts. Thus, rabbit myocardium can be considered a reliable model for electrophysiological studies, especially when generation and propagation of the impulse is studied. It may be concluded that rabbit isolated heart is more suitable model for electrophysiological studies in the isolated heart model than guinea pig heart.

Anglický abstrakt

Voltage-sensitive dye (VSD) di-4-ANEPPS is currently used in our laboratory for recording of monophasic action potentials (MAPs) in isolated hearts. In this study, its electrophysiological effects on rabbit and guinea pig myocardium were compared during staining with VSD and washout from it. Electrograms were recorded and heart rate changes and the types and frequency of arrhythmias were evaluated. Althought certain electrophysiological changes were present in the hearts of both species, these changes are mostly reversible, especially in rabbit hearts. Thus, rabbit myocardium can be considered a reliable model for electrophysiological studies, especially when generation and propagation of the impulse is studied. It may be concluded that rabbit isolated heart is more suitable model for electrophysiological studies in the isolated heart model than guinea pig heart.

Klíčová slova

Voltage-sensitive dye (VSD) di-4-ANEPPS, electrogram

Klíčová slova v angličtině

Voltage-sensitive dye (VSD) di-4-ANEPPS, electrogram

Autoři

FIALOVÁ, K.; KOLÁŘOVÁ, J.; SEKORA, J.; TANNENBERG, M.; NOVÁKOVÁ, M.; PROVAZNÍK, I.

Rok RIV

2011

Vydáno

30.06.2010

Nakladatel

Brno University of Technology

Místo

Brno

ISBN

978-80-214-4106-4

Kniha

Proceedings of Biosignal 2010: Analysis of Biomedical Signals and Images

Strany od

108

Strany do

113

Strany počet

6

BibTex

@inproceedings{BUT35735,
  author="Kateřina {Fialová} and Jana {Kolářová} and Jiří {Sekora} and Milan {Tannenberg} and Marie {Nováková} and Valentýna {Provazník}",
  title="Electrophysiological effects of Voltage-Sensitive Dye di-4-ANEPPS in Rabbit and Guinea Pig Isolated Heart",
  booktitle="Proceedings of Biosignal 2010: Analysis of Biomedical Signals and Images",
  year="2010",
  number="20",
  pages="108--113",
  publisher="Brno University of Technology",
  address="Brno",
  isbn="978-80-214-4106-4"
}