Aminated graphene oxide-inspired antibacterial and antioxidant potency of aminated chitosan bio-composite membrane toward biomedical applications: Integrated in vitro and molecular docking analysis

Aminated graphene oxide-inspired antibacterial and antioxidant potency of aminated chitosan bio-composite membrane toward biomedical applications: Integrated in vitro and molecular docking analysis

Článek WoS

Graphene-polymer bio-composites have garnered considerable attention because of their outstanding biological and mechanical properties and environmental sustainability. This study sheds light on the development of biodegradable aminated graphene oxide (Am-GO)-reinforced aminated chitosan (Am-CS) bio-composite membranes with enhanced antibacterial and antioxidant properties. Therefore, a series of Am-GO concentrations was incorporated into Am-CS matrices and systematically analyzed via FT-IR, XRD, SEM, TEM, contact angle, zeta potential, and mechanical testing. Notably, enhanced wettability was indicated by a remarkable decline in contact angle from 58.53◦ (pure Am-CS) to a minimum of 45.50◦ (Am-GO(0.1%)@Am-CS). The membranes with 0.1% Am-GO demonstrated increased surface roughness (1 μm) and a superior tensile strength of 70 N/m2 , compared to 43.1 N/m2 for pure Am-CS. It also exhibited a higher positive surface charge (+40 mV vs. +27 mV in pure Am-CS). Antibacterial evaluations revealed marked inhibition of Pseudomonas aeruginosa (93%), Escherichia coli (90%), and Staphylococcus haemolyticus (71%) with the highest Am-GO content, compared to inhibition rates of 64%, 71%, and 41% for Am-CS membrane, respectively. The ABTS decolorization assay manifested enhanced antioxidant activity of Am-GO(0.1%)@Am-CS membrane with a radical-scavenging efficiency of 62% compared to 30% for Am-CS membrane. In vitro assays exhibited favorable protein adsorption, biodegradability, and cytocompatibility of Am-GO@Am-CS membranes toward normal human cells. Furthermore, molecular docking revealed strong interactions of Am-GO and Am-CS with bacterial targets, such as DNA gyrase and metallo-β-lactamases, corroborating the enhanced antibacterial efficacy of Am-GO@Am-CS membranes with increased Am-GO. Altogether, these findings indicate that Am-GO@Am-CS bio-composites are promising multifunctional materials for wound dressing applications.

Graphene-polymer bio-composites have garnered considerable attention because of their outstanding biological and mechanical properties and environmental sustainability. This study sheds light on the development of biodegradable aminated graphene oxide (Am-GO)-reinforced aminated chitosan (Am-CS) bio-composite membranes with enhanced antibacterial and antioxidant properties. Therefore, a series of Am-GO concentrations was incorporated into Am-CS matrices and systematically analyzed via FT-IR, XRD, SEM, TEM, contact angle, zeta potential, and mechanical testing. Notably, enhanced wettability was indicated by a remarkable decline in contact angle from 58.53◦ (pure Am-CS) to a minimum of 45.50◦ (Am-GO(0.1%)@Am-CS). The membranes with 0.1% Am-GO demonstrated increased surface roughness (1 μm) and a superior tensile strength of 70 N/m2 , compared to 43.1 N/m2 for pure Am-CS. It also exhibited a higher positive surface charge (+40 mV vs. +27 mV in pure Am-CS). Antibacterial evaluations revealed marked inhibition of Pseudomonas aeruginosa (93%), Escherichia coli (90%), and Staphylococcus haemolyticus (71%) with the highest Am-GO content, compared to inhibition rates of 64%, 71%, and 41% for Am-CS membrane, respectively. The ABTS decolorization assay manifested enhanced antioxidant activity of Am-GO(0.1%)@Am-CS membrane with a radical-scavenging efficiency of 62% compared to 30% for Am-CS membrane. In vitro assays exhibited favorable protein adsorption, biodegradability, and cytocompatibility of Am-GO@Am-CS membranes toward normal human cells. Furthermore, molecular docking revealed strong interactions of Am-GO and Am-CS with bacterial targets, such as DNA gyrase and metallo-β-lactamases, corroborating the enhanced antibacterial efficacy of Am-GO@Am-CS membranes with increased Am-GO. Altogether, these findings indicate that Am-GO@Am-CS bio-composites are promising multifunctional materials for wound dressing applications.

Aminated graphene oxide; Aminated chitosan; Membrane; Antibacterial activity; Antioxidant activity; Molecular docking simulation

Aminated graphene oxide; Aminated chitosan; Membrane; Antibacterial activity; Antioxidant activity; Molecular docking simulation

ALI, A.; ABD EL-MONAEM, E.; RAGAB, A.; OUYANG, X.; ELTAWEIL, A.; MOHY ELDIN, M.; ABDALLAH, M.; ABDEL-MOTALEB, M.; HAMED, A.; HEYDARI, A.; HASSAN, M.; OMER, A.

01.04.2026

Elsevier BV

DIAMOND AND RELATED MATERIALS

165

April

Nizozemsko

113636

19

https://www.sciencedirect.com/science/article/pii/S092596352600347X