Measurement of Anti-allergic Effects Using Flow-through Piezoelectric Biosensor Based on the Mast Cells Exocytosis

Measurement of Anti-allergic Effects Using Flow-through Piezoelectric Biosensor Based on the Mast Cells Exocytosis

Článek WoS

IgE-mediated degranulation of mast cells is involved in many allergic diseases due to the proallergic and proinflammatory substances released from granules when they become activated. The initial step of sen-sitization requires IgE antibodies to bind to high affinity FcεIR receptors. The following activation of cells becomes triggered by multivalent antigen-induced oligomerization of the IgE/FcεIR complexes. The activation reaction is accompanied withby microstructural changes of the cells which can serve as signal-generating event. To monitor such events in real time, piezoelectric (PZ) quartz crystal resonator sensor was adopted to provide semi-quantitative and qualitative information relating to the structural changes and dynamics of the process. The sensitized RBL-2H3 mast cells were cultured on poly-L-lysine coated gold surface of quartz crystal integrated in the flow cell and connected with the detector providing resonance frequency and resistance of the piezosensor. The activation of mast cells by antigen – dinitrophenyl conjugated to bovine serum albumin was continuously monitored for several hours. Fur-thermore, quercetin as the efficient inhibitor of the degranulation process was tested in micromolar con-centrations. The observed PZ response was confirmed using atomic force microscopy for imaging topog-raphy and morphological changes of the mast cells fixed at different stages of the degranulation. The results from the constructed piezoelectric biosensor proved to be sensitive, fast and label free tool for screening of anti-allergic drugs.

IgE-mediated degranulation of mast cells is involved in many allergic diseases due to the proallergic and proinflammatory substances released from granules when they become activated. The initial step of sen-sitization requires IgE antibodies to bind to high affinity FcεIR receptors. The following activation of cells becomes triggered by multivalent antigen-induced oligomerization of the IgE/FcεIR complexes. The activation reaction is accompanied withby microstructural changes of the cells which can serve as signal-generating event. To monitor such events in real time, piezoelectric (PZ) quartz crystal resonator sensor was adopted to provide semi-quantitative and qualitative information relating to the structural changes and dynamics of the process. The sensitized RBL-2H3 mast cells were cultured on poly-L-lysine coated gold surface of quartz crystal integrated in the flow cell and connected with the detector providing resonance frequency and resistance of the piezosensor. The activation of mast cells by antigen – dinitrophenyl conjugated to bovine serum albumin was continuously monitored for several hours. Fur-thermore, quercetin as the efficient inhibitor of the degranulation process was tested in micromolar con-centrations. The observed PZ response was confirmed using atomic force microscopy for imaging topog-raphy and morphological changes of the mast cells fixed at different stages of the degranulation. The results from the constructed piezoelectric biosensor proved to be sensitive, fast and label free tool for screening of anti-allergic drugs.

cell-based biosensor; degranulation; quartz crystal microbalance; atomic force microscopy

cell-based biosensor; degranulation; quartz crystal microbalance; atomic force microscopy

FOHLEROVÁ, Z.; DALIBERTO, L.; PERPETUA DEL LAMA, M.; PŘIBYL, J.; KOŠELOVÁ, Z.; SKLÁDAL, P.; NAAL, Z.; NAAL, R.

23.04.2026

MICROCHIMICA ACTA

193

338

Rakouská republika

9

https://link.springer.com/article/10.1007/s00604-026-08084-6#citeas