Detail publikačního výsledku

A previously uncharacterized Factor Associated with Metabolism and Energy (FAME/C14orf105/CCDC198/1700011H14Rik) is related to evolutionary adaptation, energy balance, and kidney physiology

Petersen, J.; Englmaier, L.; Artemov, A.V.; Poverennaya, I; Mahmoud, R.; Bouderlique, T.; Tesarova, M.; Deviatiiarov, R.; Szilvásy-Szabó, A.; Akkuratov, E.E.; Reguera, D.P.; Zeberg, H.; Kaucka, M.; Kastriti, M.E.; Krivanek, J.; Radaszkiewicz, T.; Gömöryová, K.; Knauth, S.; Potesil, D.; Zdrahal, Z.; Sri Ganji, R.; Grabowski, A.; Buhl, M.E.; Zikmund, T.; Kavkova, M.; Axelson, H.; Lindgren, D.; Kramann, D.; Kuppe, C.; Erdélyi, F.; Máté, Z.; Szabó, G.; Koehne, T.; Harkany, T.; Fried, K. et al.

Originální název

A previously uncharacterized Factor Associated with Metabolism and Energy (FAME/C14orf105/CCDC198/1700011H14Rik) is related to evolutionary adaptation, energy balance, and kidney physiology

Anglický název

A previously uncharacterized Factor Associated with Metabolism and Energy (FAME/C14orf105/CCDC198/1700011H14Rik) is related to evolutionary adaptation, energy balance, and kidney physiology

Druh

Článek WoS

Originální abstrakt

In this study we use comparative genomics to uncover a gene with uncharacterized function (1700011H14Rik/C14orf105/CCDC198), which we hereby name FAME (Factor Associated with Metabolism and Energy). We observe that FAME shows an unusually high evolutionary divergence in birds and mammals. Through the comparison of single nucleotide polymorphisms, we identify gene flow of FAME from Neandertals into modern humans. We conduct knockout experiments on animals and observe altered body weight and decreased energy expenditure in Fame knockout animals, corresponding to genome-wide association studies linking FAME with higher body mass index in humans. Gene expression and subcellular localization analyses reveal that FAME is a membrane-bound protein enriched in the kidneys. Although the gene knockout results in structurally normal kidneys, we detect higher albumin in urine and lowered ferritin in the blood. Through experimental validation, we confirm interactions between FAME and ferritin and show co-localization in vesicular and plasma membranes.

Anglický abstrakt

In this study we use comparative genomics to uncover a gene with uncharacterized function (1700011H14Rik/C14orf105/CCDC198), which we hereby name FAME (Factor Associated with Metabolism and Energy). We observe that FAME shows an unusually high evolutionary divergence in birds and mammals. Through the comparison of single nucleotide polymorphisms, we identify gene flow of FAME from Neandertals into modern humans. We conduct knockout experiments on animals and observe altered body weight and decreased energy expenditure in Fame knockout animals, corresponding to genome-wide association studies linking FAME with higher body mass index in humans. Gene expression and subcellular localization analyses reveal that FAME is a membrane-bound protein enriched in the kidneys. Although the gene knockout results in structurally normal kidneys, we detect higher albumin in urine and lowered ferritin in the blood. Through experimental validation, we confirm interactions between FAME and ferritin and show co-localization in vesicular and plasma membranes.

Klíčová slova

FAME, Evolutionary genetics, Genetic models, Kidney

Klíčová slova v angličtině

FAME, Evolutionary genetics, Genetic models, Kidney

Autoři

Petersen, J.; Englmaier, L.; Artemov, A.V.; Poverennaya, I; Mahmoud, R.; Bouderlique, T.; Tesarova, M.; Deviatiiarov, R.; Szilvásy-Szabó, A.; Akkuratov, E.E.; Reguera, D.P.; Zeberg, H.; Kaucka, M.; Kastriti, M.E.; Krivanek, J.; Radaszkiewicz, T.; Gömöryová, K.; Knauth, S.; Potesil, D.; Zdrahal, Z.; Sri Ganji, R.; Grabowski, A.; Buhl, M.E.; Zikmund, T.; Kavkova, M.; Axelson, H.; Lindgren, D.; Kramann, D.; Kuppe, C.; Erdélyi, F.; Máté, Z.; Szabó, G.; Koehne, T.; Harkany, T.; Fried, K. et al.

Rok RIV

2024

Vydáno

29.05.2023

Nakladatel

NATURE PORTFOLIO

Místo

BERLIN

ISSN

2041-1723

Periodikum

Nature Communications

Svazek

14

Číslo

3092

Stát

Spojené království Velké Británie a Severního Irska

Strany od

1

Strany do

22

Strany počet

22

URL

https://www.nature.com/articles/s41467-023-38663-7

Plný text v Digitální knihovně

http://hdl.handle.net/11012/214444

BibTex

@article{BUT185232,
  author="Petersen, J. and Englmaier, L. and Artemov, A.V. and Poverennaya, I and Mahmoud, R. and Bouderlique, T. and Tesarova, M. and Deviatiiarov, R. and Szilvásy-Szabó, A. and Akkuratov, E.E. and Reguera, D.P. and Zeberg, H. and Kaucka, M. and Kastriti, M.E. and Krivanek, J. and Radaszkiewicz, T. and Gömöryová, K. and Knauth, S. and Potesil, D. and Zdrahal, Z. and Sri Ganji, R. and Grabowski, A. and Buhl, M.E. and Zikmund, T. and Kavkova, M. and Axelson, H. and Lindgren, D. and Kramann, D. and Kuppe, C. and Erdélyi, F. and Máté, Z. and Szabó, G. and Koehne, T. and Harkany, T. and Fried, K. et al.",
  title="A previously uncharacterized Factor Associated with Metabolism and Energy (FAME/C14orf105/CCDC198/1700011H14Rik) is related to evolutionary adaptation, energy balance, and kidney physiology",
  journal="Nature Communications",
  year="2023",
  volume="14",
  number="3092",
  pages="1--22",
  doi="10.1038/s41467-023-38663-7",
  issn="2041-1723",
  url="https://www.nature.com/articles/s41467-023-38663-7"
}

Dokumenty

Petersen_Nat-Commun_2023