Characterization of upconversion nanoparticles by fluorescence spectrometry and capillary electrophoresis

Characterization of upconversion nanoparticles by fluorescence spectrometry and capillary electrophoresis

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Upconversion nanoparticles (UCNPs) are a novel class of luminescent tags for applications in life and material sciences. Unlike traditional fluorophores, UCNPs exhibit emission of shorter wavelength under near-infrared excitation (typically 980 nm). In this work, we have examined these unique photophysical properties by fluorescence spectrometry and capillary electrophoresis. UCNPs co-doped with Yb(III) and Er(III) were characterized using laboratory-made fluorescence spectrometer. We have exploited and evaluated two excitation sources and the dependence of the fluorescence of UCNPs on the relative excitation power. Moreover, capillary electrophoresis with laser-induced fluorescence (CE-LIF) detection was for the first time used for characterization of the nanoparticles. It was proved that CE-LIF is a valuable method to be used for investigation of upconversion luminiscence and monitoring of the interactions of UCNPs with other molecules of interest.

Upconversion nanoparticles (UCNPs) are a novel class of luminescent tags for applications in life and material sciences. Unlike traditional fluorophores, UCNPs exhibit emission of shorter wavelength under near-infrared excitation (typically 980 nm). In this work, we have examined these unique photophysical properties by fluorescence spectrometry and capillary electrophoresis. UCNPs co-doped with Yb(III) and Er(III) were characterized using laboratory-made fluorescence spectrometer. We have exploited and evaluated two excitation sources and the dependence of the fluorescence of UCNPs on the relative excitation power. Moreover, capillary electrophoresis with laser-induced fluorescence (CE-LIF) detection was for the first time used for characterization of the nanoparticles. It was proved that CE-LIF is a valuable method to be used for investigation of upconversion luminiscence and monitoring of the interactions of UCNPs with other molecules of interest.

Upconversion nanoparticles; UCNP; fluorescence spectrometry; capillary electrophoresis

Upconversion nanoparticles; UCNP; fluorescence spectrometry; capillary electrophoresis

VANĚČKOVÁ, T.; ZÍTKA, J.; HLAVÁČEK, A.; ADAM, V.; VACULOVIČOVÁ, M.

2019

31.12.2017

Mendel University in Brno

978-80-7509-529-9

MendelNet 2017

953

957

5

https://mendelnet.cz/artkey/mnt-201701-0113_Characterization-of-upconversion-nanoparticles-by-fluorescence-spectrometry-and-capillary-electrophoresis.php?back=/magno/mnt/2017/mn1.php?secid=4

http://hdl.handle.net/11012/84178