Detail publikačního výsledku

STUDY COVALENT INTERACTIONS FRAGMENT. DNA WITH CISPLATIN

NEJDL, L.; SOCHOR, J.; JÍLKOVÁ, E.; ŠMERKOVÁ, K.; VACULOVIČOVÁ, M.; ADAM, V.; KIZEK, R.

Originální název

STUDY COVALENT INTERACTIONS FRAGMENT. DNA WITH CISPLATIN

Anglický název

STUDY COVALENT INTERACTIONS FRAGMENT. DNA WITH CISPLATIN

Druh

Stať ve sborníku v databázi WoS či Scopus

Originální abstrakt

This work was focused on monitoring of covalent cisplatin bonding with lambda DNA fragment. The PCR fragment with a length of 498 bp and 100 uM cisplatin infusion solution was used. Interactions of DNA fragment were observed electrophoreticallyand spectrophotometrically. Experiments show, that during denaturation, cisplatin significantly reduces the melting temperature of DNA fragment. The average melting temperature was determined to 71 degrees C. After addition of cisplatin (100 uM), temperaturedecreased by 15 degrees C on average.

Anglický abstrakt

This work was focused on monitoring of covalent cisplatin bonding with lambda DNA fragment. The PCR fragment with a length of 498 bp and 100 uM cisplatin infusion solution was used. Interactions of DNA fragment were observed electrophoreticallyand spectrophotometrically. Experiments show, that during denaturation, cisplatin significantly reduces the melting temperature of DNA fragment. The average melting temperature was determined to 71 degrees C. After addition of cisplatin (100 uM), temperaturedecreased by 15 degrees C on average.

Klíčová slova

DNA; cytostatic drugs; cisplatin; temperature melting; spectrophotometry; electrophoresa; electrochemistry

Klíčová slova v angličtině

DNA; cytostatic drugs; cisplatin; temperature melting; spectrophotometry; electrophoresa; electrochemistry

Autoři

NEJDL, L.; SOCHOR, J.; JÍLKOVÁ, E.; ŠMERKOVÁ, K.; VACULOVIČOVÁ, M.; ADAM, V.; KIZEK, R.

Rok RIV

2018

Vydáno

22.11.2012

ISBN

978-80-7375-836-3

Kniha

MENDELNET 2012

Strany od

1244

Strany do

1249

Strany počet

6

BibTex

@inproceedings{BUT145754,
  author="Lukáš {Nejdl} and Jiří {Sochor} and Eva {Jílková} and Kristýna {Šmerková} and Markéta {Vaculovičová} and Vojtěch {Adam} and René {Kizek}",
  title="STUDY COVALENT INTERACTIONS FRAGMENT. DNA WITH CISPLATIN",
  booktitle="MENDELNET 2012",
  year="2012",
  pages="1244--1249",
  isbn="978-80-7375-836-3"
}