Detail publikačního výsledku

Optimization of the procedure for a ligninolytic enzymes isolation from the white-rot fungi

SOLČÁNY, V.; VRŠANSKÁ, M.; VOBĚRKOVÁ, S.

Originální název

Optimization of the procedure for a ligninolytic enzymes isolation from the white-rot fungi

Anglický název

Optimization of the procedure for a ligninolytic enzymes isolation from the white-rot fungi

Druh

Stať ve sborníku v databázi WoS či Scopus

Originální abstrakt

The white-rot fungi produce wide range of extracellular enzymes (especially ligninolytic enzymes) and they can degrade complicated and difficult degradable compounds. An immobilization of enzymes causes their stability, reusability and cheapness, so they could be effectively used in biotechnologies and other branches. In this work we focused on the optimization of isolation procedure namely precipitation the enzymes, which will be used for immobilization in the next step. 75% ammonium sulphate was used to salting the enzymes and experimentally set the optimum pH for two species of white-rot fungi – Trametes versicolor and Fomes fomentarius. Our results show that the optimum pH for precipitation by ammonium sulphate is different for used species. Consequently, pH optimum for Trametes versicolor was 6, for Fomes fomentarius was 8.

Anglický abstrakt

The white-rot fungi produce wide range of extracellular enzymes (especially ligninolytic enzymes) and they can degrade complicated and difficult degradable compounds. An immobilization of enzymes causes their stability, reusability and cheapness, so they could be effectively used in biotechnologies and other branches. In this work we focused on the optimization of isolation procedure namely precipitation the enzymes, which will be used for immobilization in the next step. 75% ammonium sulphate was used to salting the enzymes and experimentally set the optimum pH for two species of white-rot fungi – Trametes versicolor and Fomes fomentarius. Our results show that the optimum pH for precipitation by ammonium sulphate is different for used species. Consequently, pH optimum for Trametes versicolor was 6, for Fomes fomentarius was 8.

Klíčová slova

white-rot fungi; Trametes versicolor; Fomes fomentarius; protein precipitation; pH

Klíčová slova v angličtině

white-rot fungi; Trametes versicolor; Fomes fomentarius; protein precipitation; pH

Autoři

SOLČÁNY, V.; VRŠANSKÁ, M.; VOBĚRKOVÁ, S.

Rok RIV

2018

Vydáno

09.11.2016

Nakladatel

Mendel University in Brno

Místo

Brno, Czech Republic

ISBN

978-80-7509-443-8

Kniha

MendelNet2016

Strany od

1011

Strany do

1015

Strany počet

5

BibTex

@inproceedings{BUT138239,
  author="Veronika {Solčány} and Martina {Vršanská} and Stanislava {Voběrková}",
  title="Optimization of the procedure for a ligninolytic enzymes isolation from the white-rot fungi",
  booktitle="MendelNet2016",
  year="2016",
  pages="1011--1015",
  publisher="Mendel University in Brno",
  address="Brno, Czech Republic",
  isbn="978-80-7509-443-8"
}