Detail publikačního výsledku

The effect of cryopreservation on the current response of CaV 3.1 transfected HEK293 cells

SVOBODA, O.; FOHLEROVÁ, Z.; HUBÁLEK, J.

Originální název

The effect of cryopreservation on the current response of CaV 3.1 transfected HEK293 cells

Anglický název

The effect of cryopreservation on the current response of CaV 3.1 transfected HEK293 cells

Druh

Abstrakt

Originální abstrakt

The main disadvantage of long-term storage of living cells in liquid nitrogen is its relatively fast evaporation. For this reason, it is necessary to refill Dewar flask quite often. In our experiments, we used low temperature freezer (-80 °C) as more economical option to liquid nitrogen and we would like to show that there is no significant influence of long-term storage of mammalian cell-sat a low temperature. The effect of temperature was studied as the electrophysiological characteristic of the whole-cell Ca2+ current in HEK293 cells. The current responses were measured from cells stored for eight months at -80 °C and compared with previously published papers. The current traces and I-V curves showed that there are no changes in current response between cells frozen at a low temperature and previously published results. From our results can be concluded that the low temperature freezer is an adequate option for storage of mammalian cells for a couple of months.

Anglický abstrakt

The main disadvantage of long-term storage of living cells in liquid nitrogen is its relatively fast evaporation. For this reason, it is necessary to refill Dewar flask quite often. In our experiments, we used low temperature freezer (-80 °C) as more economical option to liquid nitrogen and we would like to show that there is no significant influence of long-term storage of mammalian cell-sat a low temperature. The effect of temperature was studied as the electrophysiological characteristic of the whole-cell Ca2+ current in HEK293 cells. The current responses were measured from cells stored for eight months at -80 °C and compared with previously published papers. The current traces and I-V curves showed that there are no changes in current response between cells frozen at a low temperature and previously published results. From our results can be concluded that the low temperature freezer is an adequate option for storage of mammalian cells for a couple of months.

Klíčová slova

patch clamp, current response, cell freezing, HEK293

Klíčová slova v angličtině

patch clamp, current response, cell freezing, HEK293

Autoři

SVOBODA, O.; FOHLEROVÁ, Z.; HUBÁLEK, J.

Rok RIV

2016

Vydáno

23.04.2015

Nakladatel

Vysoké učení technické v Brně, Fakulta elektrotechniky a komunikačních

Místo

Brno

ISBN

9788021421451483

Kniha

Proceedings of the 21st Student Competition Conference

Edice

1

Strany od

491

Strany do

494

Strany počet

4

BibTex

@misc{BUT114476,
  author="Ondřej {Svoboda} and Zdenka {Fohlerová} and Jaromír {Hubálek}",
  title="The effect of cryopreservation on the current response of CaV 3.1 transfected HEK293 cells",
  booktitle="Proceedings of the 21st Student Competition Conference",
  year="2015",
  series="1",
  edition="1",
  pages="491--494",
  publisher="Vysoké učení technické v Brně, Fakulta elektrotechniky a komunikačních",
  address="Brno",
  isbn="9788021421451483",
  note="Abstract"
}