Detail publikačního výsledku

Interaction study of arsenic (III and V) ions with metallothioneingene (MT2A) fragment

NEJDL, L.; SKALIČKOVÁ, S.; KUDR, J.; RUTTKAY-NEDECKÝ, B.; DOSTÁLOVÁ, S.; KREMPLOVÁ, M.; KENŠOVÁ, R.; MOULICK, A.; NOVOTNÁ, M.; ADAM, V.; KIZEK, R.

Originální název

Interaction study of arsenic (III and V) ions with metallothioneingene (MT2A) fragment

Anglický název

Interaction study of arsenic (III and V) ions with metallothioneingene (MT2A) fragment

Druh

Článek WoS

Originální abstrakt

Arsenic compounds belong to the most controversial agents concerning human health. Arsenic (As) isconsidered as a top environmental element influencing human health due to its adverse effects includ-ing cancer, diabetes, cardiovascular disease, and reproductive or developmental problems. Despite theproven mutagenic, teratogenic and carcinogenic effects, the arsenic compounds are used for centuriesto treat infectious diseases. In our work, we focused on studying of interactions of As(III) and/or As(V)with DNA. Interactions between arsenic ions and DNA were monitored by UV/vis spectrophotometryby measuring absorption and fluorescence spectra, atomic absorption spectrometry, electrochemicalmeasurements (square wave voltammetry) and agarose gel electrophoresis. Using these methods, weobserved a stable structure of DNA with As(III) within the concentration range 0.4–6.25 g mL - 1. HigherAs(III) concentration caused degradation of DNA. However, similar effects were not observed for As(V).

Anglický abstrakt

Arsenic compounds belong to the most controversial agents concerning human health. Arsenic (As) isconsidered as a top environmental element influencing human health due to its adverse effects includ-ing cancer, diabetes, cardiovascular disease, and reproductive or developmental problems. Despite theproven mutagenic, teratogenic and carcinogenic effects, the arsenic compounds are used for centuriesto treat infectious diseases. In our work, we focused on studying of interactions of As(III) and/or As(V)with DNA. Interactions between arsenic ions and DNA were monitored by UV/vis spectrophotometryby measuring absorption and fluorescence spectra, atomic absorption spectrometry, electrochemicalmeasurements (square wave voltammetry) and agarose gel electrophoresis. Using these methods, weobserved a stable structure of DNA with As(III) within the concentration range 0.4–6.25 g mL - 1. HigherAs(III) concentration caused degradation of DNA. However, similar effects were not observed for As(V).

Klíčová slova

Arsenic, Spectrometry, Electrochemistry, DNA Interaction, Anticancer drug

Klíčová slova v angličtině

Arsenic, Spectrometry, Electrochemistry, DNA Interaction, Anticancer drug

Autoři

NEJDL, L.; SKALIČKOVÁ, S.; KUDR, J.; RUTTKAY-NEDECKÝ, B.; DOSTÁLOVÁ, S.; KREMPLOVÁ, M.; KENŠOVÁ, R.; MOULICK, A.; NOVOTNÁ, M.; ADAM, V.; KIZEK, R.

Rok RIV

2016

Vydáno

01.01.2015

ISSN

0141-8130

Periodikum

International Journal of Biological Macromolecules

Svazek

72

Číslo

1

Stát

Nizozemsko

Strany od

599

Strany do

605

Strany počet

7

BibTex

@article{BUT112400,
  author="Lukáš {Nejdl} and Sylvie {Skaličková} and Jiří {Kudr} and Branislav {Ruttkay-Nedecký} and Simona {Dostálová} and Monika {Kremplová} and Renáta {Kenšová} and Amitava {Moulick} and Marie {Novotná} and Vojtěch {Adam} and René {Kizek}",
  title="Interaction study of arsenic (III and V) ions with metallothioneingene (MT2A) fragment",
  journal="International Journal of Biological Macromolecules",
  year="2015",
  volume="72",
  number="1",
  pages="599--605",
  doi="10.1016/j.ijbiomac.2014.09.001",
  issn="0141-8130"
}