The importance of experimental milieu in regulation of macrophage activation

The importance of experimental milieu in regulation of macrophage activation

Článek WoS

Background: Macrophages are important source of cytokines and other compounds, which recruit additional cells to sites of infection or tissue injury. Importantly, their functions might be directly controlled by the composition of milieu in their immediate vicinity. Therefore, the analysis of their function under different in vitro as well as simulated in vivo condition is of particular interest. Materials and methods: Mice peritoneal macrophages were stimulated with different activators (e.g. LPS, IFN-y, TNF-a and interleukins or their combination) under different experimental conditions (in vitro and simulated in vivo conditions). Consequently, the physiological functions of macrophages were assessed using different luminometric, spectrophotometric, molecular, and immunohistochemical methods. Results: Our data demonstrate that there exists crucial difference in activation of macrophages under classical in vitro and simulated in vivo conditions. These abnormalities are accompanied by significant changes in inflammatory response of macrophages as well as in activation of intracellular signaling pathways. Conclusions: The activation of mice peritoneal macrophages is dependent on the composition of milieu in their immediate vicinity and the classically used in vitro systems seems to be not suitable for correct analysis of their functions.

Background: Macrophages are important source of cytokines and other compounds, which recruit additional cells to sites of infection or tissue injury. Importantly, their functions might be directly controlled by the composition of milieu in their immediate vicinity. Therefore, the analysis of their function under different in vitro as well as simulated in vivo condition is of particular interest. Materials and methods: Mice peritoneal macrophages were stimulated with different activators (e.g. LPS, IFN-y, TNF-a and interleukins or their combination) under different experimental conditions (in vitro and simulated in vivo conditions). Consequently, the physiological functions of macrophages were assessed using different luminometric, spectrophotometric, molecular, and immunohistochemical methods. Results: Our data demonstrate that there exists crucial difference in activation of macrophages under classical in vitro and simulated in vivo conditions. These abnormalities are accompanied by significant changes in inflammatory response of macrophages as well as in activation of intracellular signaling pathways. Conclusions: The activation of mice peritoneal macrophages is dependent on the composition of milieu in their immediate vicinity and the classically used in vitro systems seems to be not suitable for correct analysis of their functions.

Macrophages, experimental milieu, activation, lipopolysacchride, Interferon-gamma

Macrophages, experimental milieu, activation, lipopolysacchride, Interferon-gamma

PEKAROVÁ, M. a kol.

2014

01.05.2013

WILEY-BLACKWELL, 111 RIVER ST, HOBOKEN 07030-5774, NJ USA

USA

0014-2972

EUROPEAN JOURNAL OF CLINICAL INVESTIGATION

43

10

Spolková republika Německo

10

10