Detail publikace

Purification and biochemical characterization of polygalacturonases produced by Aureobasidium pullulans

OMELKOVÁ, J.

Originální název

Purification and biochemical characterization of polygalacturonases produced by Aureobasidium pullulans

Typ

článek v časopise - ostatní, Jost

Jazyk

angličtina

Originální abstrakt

The extracellular polygalacturonases produced by Aureobasidium pullulans isolated from waters of the Danube river were partially purified and characterized. The pH optima of polygalacturonases produced in the first phases of cultivation (48 h) and after 10 d as well as their optima of temperature, thermal stabilities, molecular masses, isoelectric points, action pattern and ability to cleave polymeric and oligomeric substrates were compared. Polygalacturonases with a random action pattern (random cleavage of pectate forming a mixture of galactosiduronides with a lower degree of polymerization) [EC 3.2.1.15] were produced only in the first phases of growth, while exopolygalacturonases [EC 3.2.1.67] with a terminal action pattern (cleavage of pectate from the nonreducing end forming D-galactopyranuronic acid as a product) were found during the whole growth. The main enzyme form with a random action pattern was glycosylated and its active site had the arrangement described previously for the active site of polygalacturonase of phytopathogenic fungi.

Klíčová slova

Aureobasidium pullulans; exopolygalacturonase; polygalacturonase

Autoři

OMELKOVÁ, J.

Rok RIV

2005

Vydáno

31. 1. 2005

Nakladatel

VERLAG Z NATURFORSCH

ISSN

0939-5075

Periodikum

ZEITSCHRIFT FUR NATURFORSCHUNG C-A JOURNAL OF BIOSCIENCES

Ročník

2005 (3)

Číslo

60

Stát

Spolková republika Německo

Strany od

91

Strany do

96

Strany počet

7

BibTex

@article{BUT46093,
  author="Jiřina {Omelková}",
  title="Purification and biochemical characterization of polygalacturonases produced by Aureobasidium pullulans",
  journal="ZEITSCHRIFT FUR NATURFORSCHUNG C-A JOURNAL OF BIOSCIENCES",
  year="2005",
  volume="2005 (3)",
  number="60",
  pages="91--96",
  issn="0939-5075"
}