Publication result detail

Purification and biochemical characterization of polygalacturonases produced by Aureobasidium pullulans

OMELKOVÁ, J.

Original Title

Purification and biochemical characterization of polygalacturonases produced by Aureobasidium pullulans

English Title

Purification and biochemical characterization of polygalacturonases produced by Aureobasidium pullulans

Type

Peer-reviewed article not indexed in WoS or Scopus

Original Abstract

The extracellular polygalacturonases produced by Aureobasidium pullulans isolated from waters of the Danube river were partially purified and characterized. The pH optima of polygalacturonases produced in the first phases of cultivation (48 h) and after 10 d as well as their optima of temperature, thermal stabilities, molecular masses, isoelectric points, action pattern and ability to cleave polymeric and oligomeric substrates were compared. Polygalacturonases with a random action pattern (random cleavage of pectate forming a mixture of galactosiduronides with a lower degree of polymerization) [EC 3.2.1.15] were produced only in the first phases of growth, while exopolygalacturonases [EC 3.2.1.67] with a terminal action pattern (cleavage of pectate from the nonreducing end forming D-galactopyranuronic acid as a product) were found during the whole growth. The main enzyme form with a random action pattern was glycosylated and its active site had the arrangement described previously for the active site of polygalacturonase of phytopathogenic fungi.

English abstract

The extracellular polygalacturonases produced by Aureobasidium pullulans isolated from waters of the Danube river were partially purified and characterized. The pH optima of polygalacturonases produced in the first phases of cultivation (48 h) and after 10 d as well as their optima of temperature, thermal stabilities, molecular masses, isoelectric points, action pattern and ability to cleave polymeric and oligomeric substrates were compared. Polygalacturonases with a random action pattern (random cleavage of pectate forming a mixture of galactosiduronides with a lower degree of polymerization) [EC 3.2.1.15] were produced only in the first phases of growth, while exopolygalacturonases [EC 3.2.1.67] with a terminal action pattern (cleavage of pectate from the nonreducing end forming D-galactopyranuronic acid as a product) were found during the whole growth. The main enzyme form with a random action pattern was glycosylated and its active site had the arrangement described previously for the active site of polygalacturonase of phytopathogenic fungi.

Keywords

Aureobasidium pullulans; exopolygalacturonase; polygalacturonase

Key words in English

Aureobasidium pullulans; exopolygalacturonase; polygalacturonase

Authors

OMELKOVÁ, J.

Released

31.01.2005

Publisher

VERLAG Z NATURFORSCH

ISBN

0939-5075

Periodical

ZEITSCHRIFT FUR NATURFORSCHUNG SECTION C-A JOURNAL OF BIOSCIENCES

Volume

2005 (3)

Number

60

State

Federal Republic of Germany

Pages from

91

Pages to

96

Pages count

7

BibTex

@article{BUT46093,
  author="Jiřina {Omelková}",
  title="Purification and biochemical characterization of polygalacturonases produced by Aureobasidium pullulans",
  journal="ZEITSCHRIFT FUR NATURFORSCHUNG SECTION C-A JOURNAL OF BIOSCIENCES",
  year="2005",
  volume="2005 (3)",
  number="60",
  pages="91--96",
  issn="0939-5075"
}