Detail publikačního výsledku

Fluorescence method for measuring cell proliferation by image analysis of cell confluency

BAIAZITOVA, L.; SKOPALÍK, J.; SVOBODA, O.; CHMELÍK, J.; PROVAZNÍK, I.

Originální název

Fluorescence method for measuring cell proliferation by image analysis of cell confluency

Anglický název

Fluorescence method for measuring cell proliferation by image analysis of cell confluency

Druh

Stať ve sborníku v databázi WoS či Scopus

Originální abstrakt

This work is based on application image analysis for automatic measuring cell confluency of adherent cells labeled with fluorescent dyes. In this paper an example of segmentation of fibroblasts (3T3 Cells Line) with different levels of confluency is demonstrated. The proposed algorithm can be applied to modified culture chambers and different adherent cell lines. Precise cell enumeration and viability computing can help to optimize coating and culture protocol. These parameters are important for quality of migration or perfusion assay experiments in vitro.

Anglický abstrakt

This work is based on application image analysis for automatic measuring cell confluency of adherent cells labeled with fluorescent dyes. In this paper an example of segmentation of fibroblasts (3T3 Cells Line) with different levels of confluency is demonstrated. The proposed algorithm can be applied to modified culture chambers and different adherent cell lines. Precise cell enumeration and viability computing can help to optimize coating and culture protocol. These parameters are important for quality of migration or perfusion assay experiments in vitro.

Klíčová slova

Confluency, segmentation, adherent cells, 3T3 Cells Line

Klíčová slova v angličtině

Confluency, segmentation, adherent cells, 3T3 Cells Line

Autoři

BAIAZITOVA, L.; SKOPALÍK, J.; SVOBODA, O.; CHMELÍK, J.; PROVAZNÍK, I.

Rok RIV

2018

Vydáno

30.05.2017

Nakladatel

Masarykova Univerzita - MUNI PRESS

Místo

Brno, Czech Republic

ISBN

978-80-210-8580-0

Kniha

XVII. Workshop of Physical Chemists and Electrochemists

Edice

1

Strany od

47

Strany do

48

Strany počet

2

URL

http://www.sci.muni.cz/~labifel/files/soubory/sborn%C3%ADk_2017.pdf

BibTex

@inproceedings{BUT137050,
  author="Larisa {Chmelíková} and Josef {Skopalík} and Ondřej {Svoboda} and Jiří {Chmelík} and Valentýna {Provazník}",
  title="Fluorescence method for measuring cell proliferation by image analysis of cell confluency",
  booktitle="XVII. Workshop of Physical Chemists and Electrochemists",
  year="2017",
  series="1",
  number="1",
  pages="47--48",
  publisher="Masarykova Univerzita - MUNI PRESS",
  address="Brno, Czech Republic",
  isbn="978-80-210-8580-0",
  url="http://www.sci.muni.cz/~labifel/files/soubory/sborn%C3%ADk_2017.pdf"
}