Publication result detail

Fluorescence method for measuring cell proliferation by image analysis of cell confluency

BAIAZITOVA, L.; SKOPALÍK, J.; SVOBODA, O.; CHMELÍK, J.; PROVAZNÍK, I.

Original Title

Fluorescence method for measuring cell proliferation by image analysis of cell confluency

English Title

Fluorescence method for measuring cell proliferation by image analysis of cell confluency

Type

Paper in proceedings (conference paper)

Original Abstract

This work is based on application image analysis for automatic measuring cell confluency of adherent cells labeled with fluorescent dyes. In this paper an example of segmentation of fibroblasts (3T3 Cells Line) with different levels of confluency is demonstrated. The proposed algorithm can be applied to modified culture chambers and different adherent cell lines. Precise cell enumeration and viability computing can help to optimize coating and culture protocol. These parameters are important for quality of migration or perfusion assay experiments in vitro.

English abstract

This work is based on application image analysis for automatic measuring cell confluency of adherent cells labeled with fluorescent dyes. In this paper an example of segmentation of fibroblasts (3T3 Cells Line) with different levels of confluency is demonstrated. The proposed algorithm can be applied to modified culture chambers and different adherent cell lines. Precise cell enumeration and viability computing can help to optimize coating and culture protocol. These parameters are important for quality of migration or perfusion assay experiments in vitro.

Keywords

Confluency, segmentation, adherent cells, 3T3 Cells Line

Key words in English

Confluency, segmentation, adherent cells, 3T3 Cells Line

Authors

BAIAZITOVA, L.; SKOPALÍK, J.; SVOBODA, O.; CHMELÍK, J.; PROVAZNÍK, I.

RIV year

2018

Released

30.05.2017

Publisher

Masarykova Univerzita - MUNI PRESS

Location

Brno, Czech Republic

ISBN

978-80-210-8580-0

Book

XVII. Workshop of Physical Chemists and Electrochemists

Edition

1

Pages from

47

Pages to

48

Pages count

2

URL

http://www.sci.muni.cz/~labifel/files/soubory/sborn%C3%ADk_2017.pdf

BibTex

@inproceedings{BUT137050,
  author="Larisa {Chmelíková} and Josef {Skopalík} and Ondřej {Svoboda} and Jiří {Chmelík} and Valentýna {Provazník}",
  title="Fluorescence method for measuring cell proliferation by image analysis of cell confluency",
  booktitle="XVII. Workshop of Physical Chemists and Electrochemists",
  year="2017",
  series="1",
  number="1",
  pages="47--48",
  publisher="Masarykova Univerzita - MUNI PRESS",
  address="Brno, Czech Republic",
  isbn="978-80-210-8580-0",
  url="http://www.sci.muni.cz/~labifel/files/soubory/sborn%C3%ADk_2017.pdf"
}