Detail publikace

Identification of viable Lactobacillus cells in fermented dairy products

TRACHTOVÁ, Š. ŠPANOVÁ, A. RITTICH, B.

Originální název

Identification of viable Lactobacillus cells in fermented dairy products

Typ

článek v časopise - ostatní, Jost

Jazyk

angličtina

Originální abstrakt

The combination PCR and ethidium monoazide (EMA-PCR) is a new metod for selective distinction between viable and dead bacterial cells. The general application of EMA is based on penetration into 'dead' cells with compromised membráně (cell wall systems) integrity. DNA bound to EMA cannot be PCR amplified. Live cells remain intact and only DNA from viable cells can be amplified. Experiments were performed using probiotic bacterial strain Lactobacillus paracasei subsp. paracasei CCDM 211/06 of human origin. The experimental conditions (time of cell incubation with EMA, and cell irradiation) were optimized. In the next step instead for live-dead cell distinction propidium monoazide was used. The Lactobacillus species specific primers were used for DNA amplification. The PCR products (250 bp) were separated using electrophoresis in 1.8 % agarose gel. The method was applied for live and dead cells identification in milk product (yoghurt).

Klíčová slova

ethidium monoazide, viable and dead bacterial cells

Autoři

TRACHTOVÁ, Š.; ŠPANOVÁ, A.; RITTICH, B.

Rok RIV

2008

Vydáno

9. 9. 2008

Nakladatel

Chemické listy

Místo

Brno

ISSN

1213-7103

Periodikum

Chemické listy

Ročník

102

Číslo

15

Stát

Česká republika

Strany od

805

Strany do

806

Strany počet

2

BibTex

@article{BUT46735,
  author="Štěpánka {Trachtová} and Alena {Španová} and Bohuslav {Rittich}",
  title="Identification of viable Lactobacillus cells in fermented dairy products",
  journal="Chemické listy",
  year="2008",
  volume="102",
  number="15",
  pages="805--806",
  issn="1213-7103"
}