Detail publikace

Determination of Renal Distribution of Zinc, Copper, Iron, and Platinum in Mouse Kidney Using LA-ICP-MS

ŠTĚPKA, P. KRATOCHVÍLOVÁ, M. KUCHYNKA, M. RAUDENSKÁ, M. HOLCOVA POLANSKA, H. VIČAR, T. VACULOVIC, T. VACULOVIČOVÁ, M. MASAŘÍK, M.

Originální název

Determination of Renal Distribution of Zinc, Copper, Iron, and Platinum in Mouse Kidney Using LA-ICP-MS

Typ

článek v časopise ve Web of Science, Jimp

Jazyk

angličtina

Originální abstrakt

The main dose-limiting side effect of cisplatin is nephrotoxicity. The utilization of cisplatin is an issue of balancing tumour toxicity versus platinum-induced nephrotoxicity. In this study, we focused on intraorgan distribution of common essential trace elements zinc, copper, and iron in healthy mouse kidneys and distribution of platinum after cisplatin treatment. Renal distribution in 12 nontreated Nu-Nu mice (males) was assessed by laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS). Furthermore, 9 Nu-Nu mice were treated with cisplatin. The order of elements concentration in kidneys was as follows: Fe > Zn > Cu. All three metals showed the higher concentrations at the cortex and medulla (28.60, 3.35, and 93.83 μg/g for Zn, Cu, and Fe, respectively) and lower concentration at the pelvis and the urinary tract (20.20, 1.93, and 62.48 μg/g for Zn, Cu, and Fe, respectively). No statistically significant difference between cortex and medulla was observed for these elements. After platinum treatment, the concentration of platinum in kidneys was enhanced more than 60-times, . Platinum significantly showed the highest accumulation in cortex (2.11 μg/g) with a gradient distribution. Platinum was less accumulated in medulla and pelvis than in cortex, and the lowest accumulation occurred in the urinary tract (1.13 μg/g). Image processing has been successfully utilized to colocalize metal distribution using LA-ICP-MS and histological samples images.

Klíčová slova

without keyword

Autoři

ŠTĚPKA, P.; KRATOCHVÍLOVÁ, M.; KUCHYNKA, M.; RAUDENSKÁ, M.; HOLCOVA POLANSKA, H.; VIČAR, T.; VACULOVIC, T.; VACULOVIČOVÁ, M.; MASAŘÍK, M.

Vydáno

26. 10. 2021

Nakladatel

Hindawi

ISSN

2314-6141

Periodikum

BioMed Research International

Ročník

2021

Číslo

1

Stát

Spojené státy americké

Strany od

1

Strany do

8

Strany počet

8

URL

https://www.hindawi.com/journals/bmri/2021/6800294/

Plný text v Digitální knihovně

http://hdl.handle.net/11012/203062

BibTex

@article{BUT173252,
  author="Petr {Štěpka} and Monika {Kratochvílová} and Michaela {Kuchynka} and Martina {Raudenská} and Hana {Holcova Polanska} and Tomáš {Vičar} and Tomas {Vaculovic} and Markéta {Vaculovičová} and Michal {Masařík}",
  title="Determination of Renal Distribution of Zinc, Copper, Iron, and Platinum in Mouse Kidney Using LA-ICP-MS",
  journal="BioMed Research International",
  year="2021",
  volume="2021",
  number="1",
  pages="1--8",
  doi="10.1155/2021/6800294",
  issn="2314-6141",
  url="https://www.hindawi.com/journals/bmri/2021/6800294/"
}