Detail publikace

Peptide-based electrochemical biosensors utilized for protein detection

VAŇOVÁ, V. MITREVSKA, K. MILOSAVLJEVIĆ, V. HYNEK, D. RICHTERA, L. ADAM, V.

Originální název

Peptide-based electrochemical biosensors utilized for protein detection

Typ

článek v časopise ve Web of Science, Jimp

Jazyk

angličtina

Originální abstrakt

Proteins are generally detected as biomarkers for tracing or determining various disorders in organisms. Biomarker proteins can be tracked in samples with various origins and in different concentrations, revealing whether an organism is in a healthy or unhealthy state. In regard to detection, electrochemical biosensors are a potential fusion of electronics, chemistry, and biology, allowing for fast and early point-of-care detection from a biological sample with the advantages of high sensitivity, simple construction, and easy operation. Peptides present a promising approach as a biorecognition element when connected with electrochemical biosensors. The benefits of short peptides lie mainly in their good stability and selective affinity to a target analyte. Therefore, peptide-based electrochemical biosensors (PBEBs) represent an alternative approach for the detection of different protein biomarkers. This review provides a summary of the past decade of recently proposed PBEBs designed for protein detection, dividing them according to different protein types: (i) enzyme detection, including proteases and kinases; (ii) antibody detection; and (iii) other protein detection. According to these protein types, different sensing mechanisms are discussed, such as the peptide cleavage by a proteases, phosphorylation by kinases, presence of antibodies, and exploiting of affinities; furthermore, measurements are obtained by different electrochemical methods. A discussion and comparison of various constructions, modifications, immobilization strategies and different sensing techniques in terms of high sensitivity, selectivity, repeatability, and potential for practical application are presented.

Klíčová slova

Proteins; Biomarkers; Electrochemistry; Biosensors; Peptides; Biorecognition element

Autoři

VAŇOVÁ, V.; MITREVSKA, K.; MILOSAVLJEVIĆ, V.; HYNEK, D.; RICHTERA, L.; ADAM, V.

Vydáno

15. 5. 2021

ISSN

0956-5663

Periodikum

BIOSENSORS & BIOELECTRONICS

Ročník

180

Číslo

113087

Stát

Nizozemsko

Strany od

1

Strany do

19

Strany počet

19

URL

https://www.sciencedirect.com/science/article/pii/S095656632100124X

BibTex

@article{BUT171293,
  author="VAŇOVÁ, V. and MITREVSKA, K. and MILOSAVLJEVIĆ, V. and HYNEK, D. and RICHTERA, L. and ADAM, V.",
  title="Peptide-based electrochemical biosensors utilized for protein detection",
  journal="BIOSENSORS & BIOELECTRONICS",
  year="2021",
  volume="180",
  number="113087",
  pages="1--19",
  doi="10.1016/j.bios.2021.113087",
  issn="0956-5663",
  url="https://www.sciencedirect.com/science/article/pii/S095656632100124X"
}