Detail publikace

Bioconjugation of upconversion nanoparticle characterized by capillary electrophoresis

VANĚČKOVÁ, T. ŠMERKOVÁ, K. ZÍTKA, J. HYNEK, D. VACULOVIČOVÁ, M. NEJDL, L.

Originální název

Bioconjugation of upconversion nanoparticle characterized by capillary electrophoresis

Typ

abstrakt

Jazyk

angličtina

Originální abstrakt

Upconversion nanoparticles (UCNPs) are an emerging class of optical materials with high potential in bioimaging due to practically no background signal and high penetration depth. Their excellent optical properties and easy surface functionalization make them perfect for conjugation with targeting ligands. In this work, capillary electrophoretic (CE) method with laserinduced fluorescence detection was used to investigate the behavior of carboxy-silica-UCNPs. Folic acid, targeting folate receptor overexpressed by wide variety of cancer cells, was used for illustrative purposes and assessed by CE under optimized conditions. Despite the numerous advantages of CE, this is the first time that CE was employed for characterization of UCNPs and their bioconjugates. The separation conditions were optimized including the background electrolyte concentration and pH. The optimized electrolyte was 20 mM borate buffer with pH 8.

Klíčová slova

Upconversion nanoparticles (UCNPs); Capillary electrophoresis

Autoři

VANĚČKOVÁ, T.; ŠMERKOVÁ, K.; ZÍTKA, J.; HYNEK, D.; VACULOVIČOVÁ, M.; NEJDL, L.

Vydáno

18. 5. 2018

ISBN

978-88-941816-1-6

Kniha

42nd International Symposium on Capillary Chromatography and 15th GC×GC Symposium ABSTRACT BOOK

Strany od

266

Strany do

266

Strany počet

1

BibTex

@misc{BUT147920,
  author="Tereza {Vaněčková} and Kristýna {Šmerková} and Jan {Zítka} and David {Hynek} and Markéta {Vaculovičová} and Lukáš {Nejdl}",
  title="Bioconjugation of upconversion nanoparticle characterized by capillary electrophoresis",
  booktitle="42nd International Symposium on Capillary Chromatography and 15th GC×GC Symposium
ABSTRACT BOOK",
  year="2018",
  pages="266--266",
  isbn="978-88-941816-1-6",
  note="abstract"
}