Detail publikace

Soluble collagen dissolution and assembling in pressurized carbon dioxide water solutions

ZUBAL, L. BONANI, W. MANIGLIO, D. CECCATO, R. RENČIUK, D. HAMPL, A. MIGLIARESI, C. JANČÁŘ, J. VOJTOVÁ, L.

Originální název

Soluble collagen dissolution and assembling in pressurized carbon dioxide water solutions

Anglický název

Soluble collagen dissolution and assembling in pressurized carbon dioxide water solutions

Jazyk

en

Originální abstrakt

Dissolution and gelation procedures have a great influence on gelation time, microstructure and mechanical properties of reconstituted collagen products. We have investigated the dissolution of atelocollagen in CO2/water solutions at low temperature (4 degrees C) at different CO2 pressures (0.3-0.9 MPa), as well as gelation kinetics and physico-chemical properties of the hydrogel obtained after CO2 removal. Compared to conventional methods, the CO2-assisted technique resulted in faster soluble collagen dissolution and faster gelation into transparent gels characterized by thin 10 nm fibrils. Electrophoresis and CD spectroscopy demonstrated that the process did not denature the soluble collagen. The possibility to obtain collagen dissolution and gelation without the use of chemical agent other than water and CO2 makes this process particularly appealing for biomedical applications.

Anglický abstrakt

Dissolution and gelation procedures have a great influence on gelation time, microstructure and mechanical properties of reconstituted collagen products. We have investigated the dissolution of atelocollagen in CO2/water solutions at low temperature (4 degrees C) at different CO2 pressures (0.3-0.9 MPa), as well as gelation kinetics and physico-chemical properties of the hydrogel obtained after CO2 removal. Compared to conventional methods, the CO2-assisted technique resulted in faster soluble collagen dissolution and faster gelation into transparent gels characterized by thin 10 nm fibrils. Electrophoresis and CD spectroscopy demonstrated that the process did not denature the soluble collagen. The possibility to obtain collagen dissolution and gelation without the use of chemical agent other than water and CO2 makes this process particularly appealing for biomedical applications.

Plný text v Digitální knihovně

Dokumenty

BibTex


@article{BUT146700,
  author="Lukáš {Zubal} and Walter {Bonani} and Devid {Maniglio} and Riccardo {Ceccato} and Daniel {Renčiuk} and Aleš {Hampl} and Claudio {Migliaresi} and Josef {Jančář} and Lucy {Vojtová}",
  title="Soluble collagen dissolution and assembling in pressurized carbon dioxide water solutions",
  annote="Dissolution and gelation procedures have a great influence on gelation time, microstructure and mechanical properties of reconstituted collagen products. We have investigated the dissolution of atelocollagen in CO2/water solutions at low temperature (4 degrees C) at different CO2 pressures (0.3-0.9 MPa), as well as gelation kinetics and physico-chemical properties of the hydrogel obtained after CO2 removal. Compared to conventional methods, the CO2-assisted technique resulted in faster soluble collagen dissolution and faster gelation into transparent gels characterized by thin 10 nm fibrils. Electrophoresis and CD spectroscopy demonstrated that the process did not denature the soluble collagen. The possibility to obtain collagen dissolution and gelation without the use of chemical agent other than water and CO2 makes this process particularly appealing for biomedical applications.",
  address="Budapest University of Technology",
  chapter="146700",
  doi="10.3144/expresspolymlett.2018.14",
  howpublished="print",
  institution="Budapest University of Technology",
  number="2",
  volume="12",
  year="2018",
  month="february",
  pages="159--170",
  publisher="Budapest University of Technology",
  type="journal article in Web of Science"
}