Detail publikace

A 3D Microfluidic Chip for Electrochemical Detection of Hydrolysed Nucleic Bases by a Modified Glassy Carbon Electrode

VLACHOVÁ, J. TMEJOVÁ, K. KOPEL, P. KORABÍK, M. ZÍTKA, J. HYNEK, D. KYNICKÝ, J. ADAM, V. KIZEK, R.

Originální název

A 3D Microfluidic Chip for Electrochemical Detection of Hydrolysed Nucleic Bases by a Modified Glassy Carbon Electrode

Typ

článek v časopise ve Web of Science, Jimp

Jazyk

angličtina

Originální abstrakt

Modification of carbon materials, especially graphene-based materials, has wide applications in electrochemical detection such as electrochemical lab-on-chip devices. A glassy carbon electrode (GCE) modified with chemically alternated graphene oxide was used as a working electrode (glassy carbon modified by graphene oxide with sulphur containing compounds and Nafion) for detection of nucleobases in hydrolysed samples (HCl pH = 2.9, 100 °C, 1 h, neutralization by NaOH). It was found out that modification, especially with trithiocyanuric acid, increased the sensitivity of detection in comparison with pure GCE. All processes were finally implemented in a microfluidic chip formed with a 3D printer by fused deposition modelling technology. As a material for chip fabrication, acrylonitrile butadiene styrene was chosen because of its mechanical and chemical stability. The chip contained the one chamber for the hydrolysis of the nucleic acid and another for the electrochemical detection by the modified GCE. This chamber was fabricated to allow for replacement of the GCE.

Klíčová slova

REDUCED GRAPHENE OXIDE; TRITHIOCYANURIC ACID; 2,4,6-TRIMERCAPTO-1,3,5-TRIAZINE TMT; FUNCTIONALIZED GRAPHENE; COMPLEXES; CHEMISTRY; SALT; 1,3,5-TRIAZINE-2,4,6-TRITHIOL; TRIMERCAPTOTRIAZINE; INHIBITION

Autoři

VLACHOVÁ, J.; TMEJOVÁ, K.; KOPEL, P.; KORABÍK, M.; ZÍTKA, J.; HYNEK, D.; KYNICKÝ, J.; ADAM, V.; KIZEK, R.

Rok RIV

2015

Vydáno

1. 2. 2015

Nakladatel

MDPI AG, POSTFACH, CH-4005 BASEL, SWITZERLAND

Místo

BASEL

ISSN

1424-8220

Periodikum

SENSORS

Ročník

15

Číslo

2

Stát

Švýcarská konfederace

Strany od

2438

Strany do

2452

Strany počet

15

URL

http://www.mdpi.com/1424-8220/15/2/2438

Plný text v Digitální knihovně

http://hdl.handle.net/11012/63725

BibTex

@article{BUT123331,
  author="Jana {Vlachová} and Kateřina {Tmejová} and Pavel {Kopel} and Michal {Korabík} and Jan {Zítka} and David {Hynek} and Jindřich {Kynický} and Vojtěch {Adam} and René {Kizek}",
  title="A 3D Microfluidic Chip for Electrochemical Detection of Hydrolysed Nucleic Bases by a Modified Glassy Carbon Electrode",
  journal="SENSORS",
  year="2015",
  volume="15",
  number="2",
  pages="2438--2452",
  doi="10.3390/s150202438",
  issn="1424-8220",
  url="http://www.mdpi.com/1424-8220/15/2/2438"
}