Detail publikace

Paramagnetic Particles and PNA Probe for Automated Separation and Electrochemical Detection of Influenza

KREJČOVÁ, L. NGUYEN, H. HYNEK, D. GURÁŇ, R. ADAM, V. KIZEK, R.

Originální název

Paramagnetic Particles and PNA Probe for Automated Separation and Electrochemical Detection of Influenza

Typ

článek v časopise ve Web of Science, Jimp

Jazyk

angličtina

Originální abstrakt

Considerable efforts have been devoted to the development of rapid and sensitive methods allowing the detection of viral nucleic acid. We herein describe an assay for identification of a specific influenza sequence. The suggested method was based on isolation using paramagnetic particles coupled with electrochemical detection of isolated product. Peptide nucleic acid (PNA) was used as a probe for hybridization and identification of the influenza-derived specific sequence. The use of PNA can show numerous benefits: PNA probe is not degradable by enzymes and the duplex of PNA with RNA/DNA is more thermostable and more resistant to pH changes than DNA/DNA or RNA/ RNA duplexes. This PNA probe assay can be applied as a magnetically guidable tool for detection of DNA/RNA samples under different conditions.

Klíčová slova

Protein nucleic acid, Biosensor, Magneticparticle- based separation, Voltammetry

Autoři

KREJČOVÁ, L.; NGUYEN, H.; HYNEK, D.; GURÁŇ, R.; ADAM, V.; KIZEK, R.

Rok RIV

2014

Vydáno

1. 11. 2014

ISSN

0009-5893

Periodikum

CHROMATOGRAPHIA

Ročník

77

Číslo

21-22

Stát

Spolková republika Německo

Strany od

1425

Strany do

1432

Strany počet

8

BibTex

@article{BUT112354,
  author="Ludmila {Krejčová} and Hoai Viet {Nguyen} and David {Hynek} and Roman {Guráň} and Vojtěch {Adam} and René {Kizek}",
  title="Paramagnetic Particles and PNA Probe for Automated Separation and Electrochemical Detection of Influenza",
  journal="CHROMATOGRAPHIA",
  year="2014",
  volume="77",
  number="21-22",
  pages="1425--1432",
  doi="10.1007/s10337-014-2737-2",
  issn="0009-5893"
}