Detail publikace

Use of PCR-DGGE for control of bacteria in chesses and their pickles

ČAKAJDOVÁ, M. TRACHTOVÁ, Š. MOHELSKÝ, T. NĚMEČKOVÁ, I. ŠPANOVÁ, A. RITTICH, B.

Originální název

Use of PCR-DGGE for control of bacteria in chesses and their pickles

Typ

konferenční sborník (ne článek)

Jazyk

angličtina

Originální abstrakt

Denaturation gradient gel electrophoresis (PCR-DGGE) was used for control of bacteria in brained cheeses and their pickles. Composition of polymerase chain reaction (PCR) mixture and DNA amplification conditions were optimized for PCR with CG clamp primers. Phenol extraction and magnetic microspheres were used for purification of amplicons. Different numbers and intensities of amplicons reflect different bacterial composition in tested cheeses and pickles.

Klíčová slova

DGGE, cheeses and their pickles

Autoři

ČAKAJDOVÁ, M.; TRACHTOVÁ, Š.; MOHELSKÝ, T.; NĚMEČKOVÁ, I.; ŠPANOVÁ, A.; RITTICH, B.

Vydáno

20. 10. 2014

Nakladatel

Institute of Analytical Chemistry AS CR, v. v. i., Brno

Místo

Brno, Czech Republic

ISBN

978-80-904959-2-0

Kniha

CECE 2014 11th International Interdisciplinary Meeting of Bioanalysis

Strany od

147

Strany do

150

Strany počet

4

BibTex

@proceedings{BUT111768,
  editor="Martina {Čakajdová} and Štěpánka {Trachtová} and Tomáš {Mohelský} and Irena {Němečková} and Alena {Španová} and Bohuslav {Rittich}",
  title="Use of PCR-DGGE for control of bacteria in chesses and their pickles",
  year="2014",
  pages="147--150",
  publisher="Institute of Analytical Chemistry AS CR, v. v. i., Brno",
  address="Brno, Czech Republic",
  isbn="978-80-904959-2-0"
}