Detail publikace

Electrochemical Microarray for Identification Pathogens: A Review

MERLOS RODRIGO, M. ZÍTKA, O. KREJČOVÁ, L. HYNEK, D. MASAŘÍK, M. KYNICKÝ, J. HEGER, Z. ADAM, V. KIZEK, R.

Originální název

Electrochemical Microarray for Identification Pathogens: A Review

Typ

článek v časopise ve Web of Science, Jimp

Jazyk

angličtina

Originální abstrakt

Numerous analytical methods are used to detect pathogens agents including methods based on the direct isolation followed by real time-polymerase chain reaction, or immunology tests. There have not been published numerous papers on the using of electrochemical microarrays for detection of viruses and/or bacteria but there is a great potential. Electrochemical detection has been developed and assay performances studied for the CombiMatrix oligonucleotide microarray platform. CombiMatrix core technology is based on a specially modified semiconductor adapted for biological applications, which contains arrays of platinum microelectrodes. The ElectraSenseTM principle is the detection of redox active chemistries proximal to specific electrodes. The target molecules are labelled with a redox enzyme via biotin–streptavidin (or avidin) interaction. Enzymatic oxidation of an electron donor substrate then occurs. The detection current is generated due to electro-reduction of the enzymatic reaction product. Thus, the ElectraSense platform has been used to develop nucleic acid assays for highly accurate genotyping of a variety of pathogens including bio-threat agents. In this review we summarize recent studies on the detection and identification of pathogens by electrochemical microarrays technology and discuss the advantages and disadvantages

Klíčová slova

Electrochemical detection; Microarray platform; Viruses, Influenza virus; Human papillomavirus.

Autoři

MERLOS RODRIGO, M.; ZÍTKA, O.; KREJČOVÁ, L.; HYNEK, D.; MASAŘÍK, M.; KYNICKÝ, J.; HEGER, Z.; ADAM, V.; KIZEK, R.

Rok RIV

2014

Vydáno

1. 7. 2014

ISSN

1452-3981

Periodikum

INTERNATIONAL JOURNAL OF ELECTROCHEMICAL SCIENCE

Ročník

9

Číslo

7

Stát

Srbská republika

Strany od

3431

Strany do

3439

Strany počet

9

BibTex

@article{BUT109363,
  author="Miguel Ángel {Merlos Rodrigo} and Ondřej {Zítka} and Ludmila {Krejčová} and David {Hynek} and Michal {Masařík} and Jindřich {Kynický} and Zbyněk {Heger} and Vojtěch {Adam} and René {Kizek}",
  title="Electrochemical Microarray for Identification Pathogens: A Review",
  journal="INTERNATIONAL JOURNAL OF ELECTROCHEMICAL SCIENCE",
  year="2014",
  volume="9",
  number="7",
  pages="3431--3439",
  issn="1452-3981"
}