Detail publikačního výsledku

Advantages and progress in the analysis of DNA by using mercury and amalgam electrodes - Review

HYNEK, D.; PRÁŠEK, J.; KOUDELKA, P.; CHOMOUCKÁ, J.; TRNKOVÁ, L.; ADAM, V.; HUBÁLEK, J.; KIZEK, R.

Originální název

Advantages and progress in the analysis of DNA by using mercury and amalgam electrodes - Review

Anglický název

Advantages and progress in the analysis of DNA by using mercury and amalgam electrodes - Review

Druh

Článek recenzovaný mimo WoS a Scopus

Originální abstrakt

The electroactivity of DNA was discovered in 1958 by Emil Palecek. Since than, there have been done great progress and development in electrochemistry of nucleic acids at various electrodes. In this review, after brief overview of milestones in research in electrochemistry, history of electroanalysis of DNA follows. Then, the attention is aimed at various electrochemical methods using a mercury electrode as a working one including linear sweep and cyclic polarography/voltammetry (elimination polarography/voltammetry), differential pulse polarography/voltammetry, square wave polarography/voltammetry, AC polarography/voltammetry and chronopotentiometry for analysis of DNA. Coupling of adsorptive transfer stripping technique to the above-mentioned methods is very promising for nucleic acid studying and is discussed. Further, advantages of mercury and amalgam electrodes are mentioned. The advantages of coupling of electroanalysis of DNA at mercury electrodes with paramagnetic particles based isolation of target molecules are shown.

Anglický abstrakt

The electroactivity of DNA was discovered in 1958 by Emil Palecek. Since than, there have been done great progress and development in electrochemistry of nucleic acids at various electrodes. In this review, after brief overview of milestones in research in electrochemistry, history of electroanalysis of DNA follows. Then, the attention is aimed at various electrochemical methods using a mercury electrode as a working one including linear sweep and cyclic polarography/voltammetry (elimination polarography/voltammetry), differential pulse polarography/voltammetry, square wave polarography/voltammetry, AC polarography/voltammetry and chronopotentiometry for analysis of DNA. Coupling of adsorptive transfer stripping technique to the above-mentioned methods is very promising for nucleic acid studying and is discussed. Further, advantages of mercury and amalgam electrodes are mentioned. The advantages of coupling of electroanalysis of DNA at mercury electrodes with paramagnetic particles based isolation of target molecules are shown.

Klíčová slova

DNA, mercury electrode, DNA hybridization, nucleic acid, reduction of nucleobases, genosensors, biosensors, nanomaterials, magnetic beads, nanotechnology, automation

Klíčová slova v angličtině

DNA, mercury electrode, DNA hybridization, nucleic acid, reduction of nucleobases, genosensors, biosensors, nanomaterials, magnetic beads, nanotechnology, automation

Autoři

HYNEK, D.; PRÁŠEK, J.; KOUDELKA, P.; CHOMOUCKÁ, J.; TRNKOVÁ, L.; ADAM, V.; HUBÁLEK, J.; KIZEK, R.

Rok RIV

2012

Vydáno

24.05.2011

Nakladatel

Bentham Science Publishers Ltd.

ISSN

1877-9476

Periodikum

Current Physical Chemistry

Svazek

1

Číslo

4

Stát

Nizozemsko

Strany od

299

Strany do

324

Strany počet

26

BibTex

@article{BUT72550,
  author="HYNEK, D. and PRÁŠEK, J. and KOUDELKA, P. and CHOMOUCKÁ, J. and TRNKOVÁ, L. and ADAM, V. and HUBÁLEK, J. and KIZEK, R.",
  title="Advantages and progress in the analysis of DNA by using mercury and amalgam electrodes - Review",
  journal="Current Physical Chemistry",
  year="2011",
  volume="1",
  number="4",
  pages="299--324",
  issn="1877-9476"
}