Detail publikačního výsledku

DNA Methylation Detection With Pacific Biosciences sequencing and Oxford Nanopore sequencing Technology

UMAIR, M.; SEDLÁŘ, K.

Originální název

DNA Methylation Detection With Pacific Biosciences sequencing and Oxford Nanopore sequencing Technology

Anglický název

DNA Methylation Detection With Pacific Biosciences sequencing and Oxford Nanopore sequencing Technology

Druh

Stať ve sborníku mimo WoS a Scopus

Originální abstrakt

DNA methylation plays a crucial role in epigenetic modification which regulates gene expression and genome stability. Application of Pacific Biosciences sequencing technology (PacBio) and Oxford Nanopore sequencing technology (ONT) has shown promising approaches for detecting single base modification for DNA methylation. In this article, we analysed the base modification detection on ONT sequences and then compared it with PacBio analysed data. We found that both Nanopore and PacBio methods were effective but showed variations in their result. We analyzed the methylation pattern in Clostridium beijerinckii NRRL B-598 and found that the ONT data in combination with tombo tool can help detect modification at low coverage sites while avoiding false reading at these areas. Our study provide insight into these two approaches of DNA methylation detection, which will help in guiding the choices of appropriate method for specific research purposes.

Anglický abstrakt

DNA methylation plays a crucial role in epigenetic modification which regulates gene expression and genome stability. Application of Pacific Biosciences sequencing technology (PacBio) and Oxford Nanopore sequencing technology (ONT) has shown promising approaches for detecting single base modification for DNA methylation. In this article, we analysed the base modification detection on ONT sequences and then compared it with PacBio analysed data. We found that both Nanopore and PacBio methods were effective but showed variations in their result. We analyzed the methylation pattern in Clostridium beijerinckii NRRL B-598 and found that the ONT data in combination with tombo tool can help detect modification at low coverage sites while avoiding false reading at these areas. Our study provide insight into these two approaches of DNA methylation detection, which will help in guiding the choices of appropriate method for specific research purposes.

Klíčová slova

DNA Methylation; Clostridium beijerinckii; ONT; PacBio; 6mA

Klíčová slova v angličtině

DNA Methylation; Clostridium beijerinckii; ONT; PacBio; 6mA

Autoři

UMAIR, M.; SEDLÁŘ, K.

Vydáno

28.04.2023

ISBN

978-80-214-6153-6

Kniha

PROCEEDINGS I OF THE 29TH STUDENT EEICT 2023

Strany od

275

Strany do

278

Strany počet

4

URL

https://www.eeict.cz/eeict_download/archiv/sborniky/EEICT_2023_sbornik_1.pdf

BibTex

@inproceedings{BUT187062,
  author="{Mohammad Umair} and Karel {Sedlář}",
  title="DNA Methylation Detection With Pacific Biosciences sequencing and Oxford Nanopore sequencing Technology",
  booktitle="PROCEEDINGS I OF THE 29TH STUDENT EEICT 2023",
  year="2023",
  pages="275--278",
  isbn="978-80-214-6153-6",
  url="https://www.eeict.cz/eeict_download/archiv/sborniky/EEICT_2023_sbornik_1.pdf"
}