Detail publikačního výsledku

Gold Nanoparticles as Labels for Immunochemical Analysis Using Laser Ablation Inductively Coupled Plasma Mass Spectrometry

TVRDOŇOVÁ, M.; VLČNOVSKÁ, M.; POMPEIANO VANÍČKOVÁ, L.; KANICKÝ, V.; ADAM, V.; ASCHER, L.; JAKUBOWSKI, N.; VACULOVIČOVÁ, M.; VACULOVIČ, T.

Originální název

Gold Nanoparticles as Labels for Immunochemical Analysis Using Laser Ablation Inductively Coupled Plasma Mass Spectrometry

Anglický název

Gold Nanoparticles as Labels for Immunochemical Analysis Using Laser Ablation Inductively Coupled Plasma Mass Spectrometry

Druh

Článek WoS

Originální abstrakt

In this paper, we describe the labelling of antibodies by gold nanoparticles (AuNPs) with diameters of 10 and 60nm with detection by laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS). Additionally, the AuNPs labelling strategy is compared with commercially available labelling reagents based on MeCAT (metal coded affinity tagging). Proof of principle experiments based on dot blot experiments were performed. The two labelling methods investigated were compared by sensitivity and limit of detection (LOD). The absolute LODs achieved were in the range of tens of picograms for AuNP labelling compared to a few hundred picograms by the MeCAT labelling.

Anglický abstrakt

In this paper, we describe the labelling of antibodies by gold nanoparticles (AuNPs) with diameters of 10 and 60nm with detection by laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS). Additionally, the AuNPs labelling strategy is compared with commercially available labelling reagents based on MeCAT (metal coded affinity tagging). Proof of principle experiments based on dot blot experiments were performed. The two labelling methods investigated were compared by sensitivity and limit of detection (LOD). The absolute LODs achieved were in the range of tens of picograms for AuNP labelling compared to a few hundred picograms by the MeCAT labelling.

Klíčová slova

Dot blot; Antibody labelling; Gold nanoparticles; LA-ICP-MS; Immunochemistry

Klíčová slova v angličtině

Dot blot; Antibody labelling; Gold nanoparticles; LA-ICP-MS; Immunochemistry

Autoři

TVRDOŇOVÁ, M.; VLČNOVSKÁ, M.; POMPEIANO VANÍČKOVÁ, L.; KANICKÝ, V.; ADAM, V.; ASCHER, L.; JAKUBOWSKI, N.; VACULOVIČOVÁ, M.; VACULOVIČ, T.

Rok RIV

2019

Vydáno

31.01.2019

ISSN

1618-2642

Periodikum

ANALYTICAL AND BIOANALYTICAL CHEMISTRY

Svazek

411

Číslo

3

Stát

Spolková republika Německo

Strany od

559

Strany do

564

Strany počet

6

BibTex

@article{BUT156134,
  author="Michaela {Tvrdoňová} and Marcela {Vlčnovská} and Lucie {Pompeiano Vaníčková} and Viktor {Kanický} and Vojtěch {Adam} and Lena {Ascher} and Norbert {Jakubowski} and Markéta {Vaculovičová} and Tomáš {Vaculovič}",
  title="Gold Nanoparticles as Labels for Immunochemical Analysis Using Laser Ablation Inductively Coupled Plasma Mass Spectrometry",
  journal="ANALYTICAL AND BIOANALYTICAL CHEMISTRY",
  year="2019",
  volume="411",
  number="3",
  pages="559--564",
  doi="10.1007/s00216-018-1300-7",
  issn="1618-2642"
}

Dokumenty

Gold Nanoparticles as Labels for Immunochemical Analysis Using Laser Ablation Inductively Coupled Plasma Mass Spectrometry