Detail publikačního výsledku

Satellite DNA and Transposable Elements in Seabuckthorn (Hippophae rhamnoides), a Dioecious Plant with Small Y and Large X Chromosomes

PUTEROVÁ, J.; RAZUMOVA, O.; MARTÍNEK, T.; ALEXANDROV, O.; DIVASHUK, M.; KUBÁT, Z.; HOBZA, R.; KARLOV, G.; KEJNOVSKÝ, E.

Originální název

Satellite DNA and Transposable Elements in Seabuckthorn (Hippophae rhamnoides), a Dioecious Plant with Small Y and Large X Chromosomes

Anglický název

Satellite DNA and Transposable Elements in Seabuckthorn (Hippophae rhamnoides), a Dioecious Plant with Small Y and Large X Chromosomes

Druh

Článek WoS

Originální abstrakt


Seabuckthorn (Hippophae rhamnoides) is a dioecious shrub commonly used in the pharmaceutical, cosmetic, and environmental
industry as a source of oil, minerals and vitamins. In this study, we analyzed the transposable elements and satellites in its genome.We
carried out Illumina DNA sequencing and reconstructed the main repetitive DNA sequences. For data analysis, we developed a new
bioinformatics approach for advanced satellite DNA analysis and showed that about 25% of the genome consists of satellite DNA and
about 24% is formed of transposable elements, dominated by Ty3/Gypsy and Ty1/Copia LTR retrotransposons. FISH mapping
revealed X chromosome-accumulated, Y chromosome-specific or both sex chromosomes-accumulated satellites but most satellites
were found on autosomes. Transposable elements were located mostly in the subtelomeres of all chromosomes. The 5S rDNA and
45S rDNA were localized on one autosomal locus each. Although we demonstrated the small size of the Y chromosome of the
seabuckthorn and accumulated satellite DNA there, we were unable to estimate the age and extent of the Y chromosome degeneration.
Analysis of dioecious relatives such as Shepherdia would shed more light on the evolution of these sex chromosomes.

Anglický abstrakt


Seabuckthorn (Hippophae rhamnoides) is a dioecious shrub commonly used in the pharmaceutical, cosmetic, and environmental
industry as a source of oil, minerals and vitamins. In this study, we analyzed the transposable elements and satellites in its genome.We
carried out Illumina DNA sequencing and reconstructed the main repetitive DNA sequences. For data analysis, we developed a new
bioinformatics approach for advanced satellite DNA analysis and showed that about 25% of the genome consists of satellite DNA and
about 24% is formed of transposable elements, dominated by Ty3/Gypsy and Ty1/Copia LTR retrotransposons. FISH mapping
revealed X chromosome-accumulated, Y chromosome-specific or both sex chromosomes-accumulated satellites but most satellites
were found on autosomes. Transposable elements were located mostly in the subtelomeres of all chromosomes. The 5S rDNA and
45S rDNA were localized on one autosomal locus each. Although we demonstrated the small size of the Y chromosome of the
seabuckthorn and accumulated satellite DNA there, we were unable to estimate the age and extent of the Y chromosome degeneration.
Analysis of dioecious relatives such as Shepherdia would shed more light on the evolution of these sex chromosomes.

Klíčová slova

sex chromosomes, genome composition, chromosomal localization, repetitive DNA

Klíčová slova v angličtině

sex chromosomes, genome composition, chromosomal localization, repetitive DNA

Autoři

PUTEROVÁ, J.; RAZUMOVA, O.; MARTÍNEK, T.; ALEXANDROV, O.; DIVASHUK, M.; KUBÁT, Z.; HOBZA, R.; KARLOV, G.; KEJNOVSKÝ, E.

Rok RIV

2018

Vydáno

02.02.2017

ISSN

1759-6653

Periodikum

Genome Biology and Evolution

Svazek

9

Číslo

1

Stát

Spojené království Velké Británie a Severního Irska

Strany od

197

Strany do

212

Strany počet

16

URL

https://academic.oup.com/gbe/article/9/1/197/2830930

Plný text v Digitální knihovně

http://hdl.handle.net/11012/137198

BibTex

@article{BUT144426,
  author="Janka {Puterová} and Olga {Razumova} and Tomáš {Martínek} and Oleg {Alexandrov} and Mikhail {Divashuk} and Zdeněk {Kubát} and Roman {Hobza} and Gennady {Karlov} and Eduard {Kejnovský}",
  title="Satellite DNA and Transposable Elements in Seabuckthorn (Hippophae rhamnoides), a Dioecious Plant with Small Y and Large X Chromosomes",
  journal="Genome Biology and Evolution",
  year="2017",
  volume="9",
  number="1",
  pages="197--212",
  doi="10.1093/gbe/evw303",
  issn="1759-6653",
  url="https://academic.oup.com/gbe/article/9/1/197/2830930"
}

Dokumenty

evw303
Satellite_DNA_popis
Satellite_DNA