Detail publikačního výsledku

Three-dimensional fluorescence lifetime imaging in confocal microscopy of living cells

BAIAZITOVA, L.; ČMIEL, V.; SKOPALÍK, J.; SVOBODA, O.; PROVAZNÍK, I.

Originální název

Three-dimensional fluorescence lifetime imaging in confocal microscopy of living cells

Anglický název

Three-dimensional fluorescence lifetime imaging in confocal microscopy of living cells

Druh

Stať ve sborníku v databázi WoS či Scopus

Originální abstrakt

Fluorescence lifetime imaging (FLIM) is a modern optical method which increases the potential of standard microscopy. This paper shows the possibilities of extended fluorescence lifetime evaluation and imaging in studying three-dimensional structures such as compartments of living cells with different fluorescence lifetimes. The method for quasi-FLIM image calculation is presented and image processing steps useful for biological experiments are suggested.

Anglický abstrakt

Fluorescence lifetime imaging (FLIM) is a modern optical method which increases the potential of standard microscopy. This paper shows the possibilities of extended fluorescence lifetime evaluation and imaging in studying three-dimensional structures such as compartments of living cells with different fluorescence lifetimes. The method for quasi-FLIM image calculation is presented and image processing steps useful for biological experiments are suggested.

Klíčová slova

Fluorescence lifetime; FLIM; cardiomyocyte; stromal cell; image segmentation

Klíčová slova v angličtině

Fluorescence lifetime; FLIM; cardiomyocyte; stromal cell; image segmentation

Autoři

BAIAZITOVA, L.; ČMIEL, V.; SKOPALÍK, J.; SVOBODA, O.; PROVAZNÍK, I.

Rok RIV

2018

Vydáno

28.08.2017

Místo

Kos island, Greece

ISBN

978-0-9928626-7-1

Kniha

2017 25th European Signal Processing Conference (EUSIPCO)

ISSN

2219-5491

Periodikum

European Signal Processing Conference EUSIPCO

Stát

Spojené státy americké

Strany od

439

Strany do

443

Strany počet

5

URL

https://ieeexplore.ieee.org/document/8081245

BibTex

@inproceedings{BUT138959,
  author="Larisa {Chmelíková} and Vratislav {Čmiel} and Josef {Skopalík} and Ondřej {Svoboda} and Valentýna {Provazník}",
  title="Three-dimensional fluorescence lifetime imaging in confocal microscopy of living cells",
  booktitle="2017 25th European Signal Processing Conference (EUSIPCO)",
  year="2017",
  journal="European Signal Processing Conference EUSIPCO",
  pages="439--443",
  address="Kos island, Greece",
  doi="10.23919/EUSIPCO.2017.8081245",
  isbn="978-0-9928626-7-1",
  issn="2219-5491",
  url="https://ieeexplore.ieee.org/document/8081245"
}