Detail publikace

Metallothionein-3 promotes cisplatin chemoresistance remodelling in neuroblastoma

MERLOS RODRIGO, M. MICHÁLKOVÁ, H. STRMISKA, V. CASAR, B. CRESPO, P. DE LOS RIOS, V. CASAL ÁLVAREZ, J. HADDAD, Y. GURÁŇ, R. ECKSCHLAGER, T. POKORNÁ, P. HEGER, Z. ADAM, V.

Originální název

Metallothionein-3 promotes cisplatin chemoresistance remodelling in neuroblastoma

Typ

článek v časopise ve Web of Science, Jimp

Jazyk

angličtina

Originální abstrakt

Metallothionein-3 has poorly characterized functions in neuroblastoma. Cisplatin-based chemotherapy is a major regimen to treat neuroblastoma, but its clinical efficacy is limited by chemoresistance. We investigated the impact of human metallothionein-3 (hMT3) up-regulation in neuroblastoma cells and the mechanisms underlying the cisplatin-resistance. We confirmed the cisplatin-metallothionein complex formation using mass spectrometry. Overexpression of hMT3 decreased the sensitivity of neuroblastoma UKF-NB-4 cells to cisplatin. We report, for the first time, cisplatin-sensitive human UKF-NB-4 cells remodelled into cisplatin-resistant cells via high and constitutive hMT3 expression in an in vivo model using chick chorioallantoic membrane assay. Comparative proteomic analysis demonstrated that several biological pathways related to apoptosis, transport, proteasome, and cellular stress were involved in cisplatin-resistance in hMT3 overexpressing UKF-NB-4 cells. Overall, our data confirmed that up-regulation of hMT3 positively correlated with increased cisplatin-chemoresistance in neuroblastoma, and a high level of hMT3 could be one of the causes of frequent tumour relapses.

Klíčová slova

Metallothionein-3; cancer; neuroblastoma; cisplatin; chemoresistance

Autoři

MERLOS RODRIGO, M.; MICHÁLKOVÁ, H.; STRMISKA, V.; CASAR, B.; CRESPO, P.; DE LOS RIOS, V.; CASAL ÁLVAREZ, J.; HADDAD, Y.; GURÁŇ, R.; ECKSCHLAGER, T.; POKORNÁ, P.; HEGER, Z.; ADAM, V.

Vydáno

9. 3. 2021

Nakladatel

Springer Nature

ISSN

2045-2322

Periodikum

Scientific Reports

Ročník

11

Číslo

1

Stát

Spojené království Velké Británie a Severního Irska

Strany od

1

Strany do

14

Strany počet

14

URL

https://www.nature.com/articles/s41598-021-84185-x

Plný text v Digitální knihovně

http://hdl.handle.net/11012/196716

BibTex

@article{BUT171008,
  author="Miguel Ángel {Merlos Rodrigo} and Hana {Michálková} and Vladislav {Strmiska} and Berta {Casar} and Piero {Crespo} and Vivian {De los Rios} and José Ignacio {Casal Álvarez} and Yazan Abdulmajeed Eyadh {Haddad} and Roman {Guráň} and Tomáš {Eckschlager} and Petra {Pokorná} and Zbyněk {Heger} and Vojtěch {Adam}",
  title="Metallothionein-3 promotes cisplatin chemoresistance remodelling in neuroblastoma",
  journal="Scientific Reports",
  year="2021",
  volume="11",
  number="1",
  pages="1--14",
  doi="10.1038/s41598-021-84185-x",
  issn="2045-2322",
  url="https://www.nature.com/articles/s41598-021-84185-x"
}