Czech

4

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The course uses teaching methods in form of Lecture - 2 teaching hours per week. The e-learning system (LMS Moodle) is available to teachers and students.

Examination is oral

1) Introduction to bioanalytical methods and their application
2) Overview of bioanalytical techniques, basic principles of biopolymer analysis
3) Enzymology - Enzyme distribution, enzyme kinetics, enzymes as analytical reagents
4) Enzymology - immobilization of enzymes, use in practice
5) Immunochemical Methods - Principles and Overview of Antibody Techniques
6) Immunochemical methods - fluorescence, bioluminescence, radioactive and enzyme immunoassay
7) Nanotechnology, biosensors, construction and applications
8) Chromatographic techniques - gel permeation, affinity and ion exchange chromatography, gas (GC) and liquid chromatography (HPLC, UHPLC), supercritical fluid chromatography (SFC) and their use in biomolecular analysis
9) Spectrophotometric and microscopic methods - optical, fluorescence, infrared, raman and high-frequency spectroscopy, nuclear magnetic resonance, atomic absorption and emission spectrometry
10) Electrophoretic methods - electrophoresis, isoelectric focusing, isotachophoresis, affinity, two-dimensional, zonal and capillary electrophoresis.
11) Molecular techniques, polymerase chain reaction (PCR), RT-PCR detection of specific product sequences in situ, DNA and RNA chips, examples of practical use
12) Biological and microbiological analytical methods, flow cytometry, fluorescence techniques, electron microscopy
13) Modern bioanalytical techniques

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The aim is to acquire knowledge about the methods that are used for analysis of biological materials.

Attendance at lectures is not obligatory but it is recommended

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Záruba, Kamil. Analytická chemie. Praha: VSCHT v Praze, 2016. (CS)
Manz, A. Bioanalytical chemistry. London: Imperial College Press, 2015. (EN)

UGO, Paolo and Coll. Bioanalytical Chemistry. De Gruyter, 2021. (EN)
Ramesh, Vasudevan. Biomolecular and Bioanalytical Techniques. Wiley, 2019. (EN)

eLearning: currently opened course

26 hours, optionally

eLearning: currently opened course