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Doctoral Thesis
Author of thesis: Ing. Nicole Talaš Černeková
Acad. year: 2025/2026
Supervisor: prof. Ing. Adriána Kovalčík, Ph.D.
Reviewers: doc. RNDr. Ondřej Sedláček, Ph.D., prof. Ing. Vladimír Sedlařík, Ph.D.
This dissertation thesis designs sustainable hydrogels with tuneable viscoelasticity, swelling and transport properties. The first part describes the preparation and characterisation of hydrogels based on agarose, gellan gum and carrageenan. Due to its low viscoelastic strength, carrageenan hydrogel was selected for further modification with two biopolymers – poly(3-hydroxybutyrate) (PHB) and lignin. Composite carrageenan hydrogels containing PHB nanoparticles, prepared via nanoprecipitation, exhibited higher viscoelastic strength, slightly reduced swelling and slower release of active substances. Lignin-modified hydrogels formed semi-interpenetrating networks with increased viscoelastic moduli and antioxidant properties. The addition of PHB or lignin allows regulation of swelling and release rates, which is valuable for biomedical applications. The next part focuses on hydrogels prepared directly from polyhydroxyalkanoates (PHAs) – PHB and its copolymer. Despite their hydrophobic nature, hydrophilic hydrogels were successfully prepared, and agarose and polyvinyl alcohol were tested as additives. These hydrogels showed high strength, flexibility and efficient absorption and release of model substances. In the final part, PHA hydrogels were coated with a layer of polypyrrole using oxidative chemical vapour deposition (oCVD). Optimisation of oxidant loading and deposition conditions yielded electrically conductive hydrogel composites. Overall, the dissertation establishes a toolbox for mechanical and transport tuning in sustainable hydrogels, spanning polysaccharide blends, PHA-based networks and oCVD-functionalised composites, highlighting their potential for controlled release, biointerfaces and soft bioelectronics.
Hydrogels; polysaccharides; viscoelasticity; nanoparticles; lignin; polyhydroxyalkanoates (PHA); release; atomic force microscopy (AFM); conductivity.
Date of defence
22.01.2026
Date of publish
21.01.2029
Result of the defence
Defended (thesis was successfully defended)
Process of defence
Obhajoba proběhla v angličtině. Předseda komise představil doktorandku a předal jí slovo. Ing. Černeková absolvovala v roce 2025 zahraniční stáž v Nizozemí. Je spoluautorkou pěti publikací, ve dvou případech je uvedena na prvním místě. Je spoluautorkou celé řady konferenčních příspěvků.
Language of thesis
English
Faculty
Fakulta chemická
Department
Institute of Food Science and Biotechnology
Study programme
Biophysical Chemistry (DKCP_BCH)
Composition of Committee
prof. Ing. Miloslav Pekař, CSc. (předseda) doc. RNDr. Ondřej Sedláček, Ph.D. (člen) prof. Ing. Vladimír Sedlařík, Ph.D. (člen) prof. Ing. Marián Lehocký, Ph.D. (člen) Ing. Adam Strachota, Ph.D. (člen) Ing. Lukáš Nejdl, Ph.D. (člen) prof. Ing. Martina Klučáková, Ph.D. (člen)
Supervisor’s reportprof. Ing. Adriána Kovalčík, Ph.D.
Reviewer’s reportdoc. RNDr. Ondřej Sedláček, Ph.D.
Reviewer’s reportprof. Ing. Vladimír Sedlařík, Ph.D.
Reasons for publication postponement
Publication of dissertation thesis is in accordance with the provision of § 47b, paragraph 4 of Act No. 111/1998 Coll., on Higher Education Institutions, postponed. The reason for postponing the publication is the fact that the dissertation thesis, contains information protected by special legal regulations (eg No. 527/1990 Coll., on Inventions and Improvement Proposals).
Responsibility: Mgr. et Mgr. Hana Odstrčilová