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Bachelor's Thesis
Author of thesis: Monika Horká
Acad. year: 2025/2026
Supervisor: prof. Ing. Adriána Kovalčík, Ph.D.
Reviewer: Ing. Zuzana Slavíková, Ph.D.
This bachelor thesis focuses on the preparation, characterization, and biological evaluation of composite nanofibrous materials with potential biomedical applications as wound dressings. Biodegradable and biocompatible polyhydroxybutyrate (PHB) was selected as the polymer matrix. To impart the necessary antimicrobial and antioxidant activity, three selected natural phenolic monomers derived from lignin—ferulic acid, coumaric acid, and vanillic acid—were incorporated into the fiber structure. In the experimental section, the absorption maxima of the pure substances in various solvents were first characterized spectrophotometrically, and their total antioxidant activity was determined using the ABTS•+ cation radical. Functionalized PHB nanofibers with a 10% addition of the active derivatives were successfully fabricated via forcespinning (centrifugal spinning) from a 4% polymer solution in chloroform. Subsequently, the encapsulation efficiency of the process was evaluated spectrophotometrically, and the sustained release kinetics of the individual active substances from the nanofibers into a PBS solution were monitored. A key outcome of this work was the determination of the antimicrobial efficacy of the prepared materials. Testing was performed using the broth dilution method and the resazurin assay against two model bacterial strains—Gram-negative Escherichia coli and Gram-positive Staphylococcus epidermidis. The results indicate that the successful encapsulation of lignin derivatives significantly modifies the properties of PHB nanofibers and paves the way for their application in the field of active healing of infected wounds.
polyhydroxybutyrate, PHB, forcespinning, lignin derivatives, ferulic acid, coumaric acid, vanillic acid, antioxidant activity, antimicrobial activity, resazurin assay, wound dressing
Date of defence
11.06.2026
Result of the defence
Defended (thesis was successfully defended)
Grading
B
Process of defence
Obhajoba proběhla podle následujícího schématu: prezentace student-vyjádření vedoucí/ho-oponentský posudek-reakce na posudek-diskuse s komisí. Studentka přednesla velmi dobrý výtah výsledků své bakalářské práce, zodpověděla dotazy oponentské i členů komise, reagovala na připomínky k bakalářské práci. V diskusi tak studentka prokázala velmi dobrou schopnost orientace v teoretických i praktických základech problematiky bakalářské práce. Komise, s uvážením hodnocení v posudku a vyjádření vedoucí/ho, zhodnotila její bakalářskou práci celkově jako velmi dobrou. Pekař: K čemu se vztahovala enkapsulační účinnost? Jak je to s hydrofobicitou, resp. rozpustností daných látek? Obruča: Jak se látky dostaly do vláken? Jak si vysvětlujete účinnost více jak 100% u antimikrobiálních testů? Kozáková: Jak se stanovovala enkapsuláční účinnost?
Language of thesis
Czech
Faculty
Fakulta chemická
Department
Institute of Physical and Applied Chemistry
Study programme
Chemistry for Medical Application (BPCP_CHMA)
Composition of Committee
prof. Ing. Miloslav Pekař, CSc. (předseda) doc. Ing. Zdenka Kozáková, Ph.D. (člen) prof. Ing. Stanislav Obruča, Ph.D. (místopředseda) doc. Ing. Vojtěch Enev, Ph.D. (člen) doc. Ing. Petr Dzik, Ph.D. (člen)
Supervisor’s reportprof. Ing. Adriána Kovalčík, Ph.D.
Grade proposed by supervisor: A
Reviewer’s reportIng. Zuzana Slavíková, Ph.D.
Grade proposed by reviewer: B
Responsibility: Mgr. et Mgr. Hana Odstrčilová