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Course detail
FEKT-MPA-MOLAcad. year: 2026/2027
The course provides an in-depth understanding of physiological and pathophysiological processes in living organisms at the molecular level. It examines the molecular differences and interactions among eukaryotic, prokaryotic, and viral systems, with a focus on key cellular processes, biomolecular mechanisms, and regulatory networks that govern life at the molecular and cellular scale. Students will gain insight into genome organization, gene expression, protein structure and function, enzymatic mechanisms, and the roles of non-coding RNAs, as well as the molecular basis of immune responses, cellular differentiation, and development.Students will acquire comprehensive theoretical knowledge and hands-on experience with modern experimental and computational techniques, including DNA/RNA analysis, protein characterization, high-throughput sequencing, omics technologies, and bioinformatic data analysis. These foundations are essential for applications in biomedicine, genetic engineering, biotechnology, and commercial bioproduction, preparing students to address contemporary challenges .
Language of instruction
Number of ECTS credits
Mode of study
Guarantor
Department
Offered to foreign students
Entry knowledge
Students should be able to explain the fundamental principles of classical genetics and demonstrate knowledge of basic concepts and laws of molecular biology. In general, knowledge at the bachelor’s degree level is expected.
Participation in laboratory work is conditional upon holding a valid qualification as a “trained person” in accordance with § 4 of Government Regulation No. 194/2022 Coll., which students must obtain prior to the start of practical sessions. Information on obtaining this qualification is provided in the Dean’s Directive “Informing Students to Safety Regulations.”
Rules for evaluation and completion of the course
The overall assessment of the course is based on a combination of continuous evaluation of laboratory exercises, a project, and an oral examination:Up to 40 points for laboratory exercises (including one test, active participation in laboratory sessions, and independent preparation)Up to 20 points for the projectUp to 40 points for the oral examinationThe oral examination evaluates students’ knowledge and comprehension of advanced concepts in molecular biology, biotechnology, and modern molecular methods.Laboratory attendance is mandatory. Legitimately excused absences may only be made up with prior approval from the instructor.
Aims
The aim of the course is to provide students with advanced knowledge of the molecular biological differences between eukaryotes, prokaryotes, and viruses, including their interactions, and to foster understanding of the principles governing cellular function and regulation. The course emphasizes an interdisciplinary approach, linking molecular biology with biomedical research and biotechnological applications, including aspects of commercial biotechnological production.Students will learn to interpret molecular processes, critically evaluate experimental data, and apply molecular biology principles in theoretical, computational, and practical contexts. The course also develops the ability to integrate knowledge from molecular biology, biotechnology, and biomedical research, work effectively in multidisciplinary teams, and address contemporary challenges in modern biomedical and biotechnological research.
Study aids
Prerequisites and corequisites
Basic literature
Recommended reading
Classification of course in study plans
specialization NBIO , 0 year of study, winter semester, elective
Lecture
Teacher / Lecturer
Syllabus
1. Origin and Evolution of Molecular and Cellular Systems2. Cellular Organization and Functional Diversity3. Biomolecular Foundations of Cellular Functions4. Genome Structure and Genetic Information5. Gene Expression and Regulatory Mechanisms6. Transcriptomics and the Biology of Non-Coding RNAs7. Microbial and Viral Systems in Host Interaction8. Developmental Biology and Stem Cell Biology9. Applied Molecular Methods I – Nucleic Acids10. Applied Molecular Methods II – Proteins and Metabolites11. Applications in Biotechnology and Biomedicine
Laboratory exercise
Project
Students will work in small teams to explore selected modern laboratory techniques used in contemporary biomedical and biological research (e.g. advanced CRISPR–Cas systems, cryo-CT microscopy, organ-on-chip technologies, and omics approaches). They will analyse a chosen method in the context of commonly used techniques, focusing on its basic principles, key technical characteristics, advantages and limitations, and areas of application in basic and applied research. Students will also compare the selected method with traditional approaches and discuss possibilities for data processing and interpretation, including relevant analytical and bioinformatic tools. Project outcomes will be presented in the form of a mini-conference, where students will present their work to instructors and peers and participate in professional discussion.
Individual preparation - working on the assigned tasks
Students are provided with learning materials for each laboratory assignment. They are expected to study these materials independently in advance, drawing on practical session presentations, resources available in the course e-learning platform, and additional relevant scholarly and scientific sources. As part of their preparation, students also undertake a project in which they analyse, develop, and present a predefined topic.
Individual preparation for a final exam