Course detail
Introduction to Molecular Biology and Genetics
FEKT-AMOLAcad. year: 2012/2013
The students are provided with a review of the latest findings in the field of molecular and cellular biology: storage and expression of genetic information, construction and function of the cell and cellular interaction. They develop skills in microscopic practice, learn the fundamentals of biological experiment and its evaluation. The acquired knowledge and skills will constitute a basis for a better understanding of other subjects – pathological physiology, introduction to clinical medicine and analysis of biological signals.
Language of instruction
Number of ECTS credits
Mode of study
Guarantor
Learning outcomes of the course unit
Prerequisites
Co-requisites
Planned learning activities and teaching methods
Assesment methods and criteria linked to learning outcomes
Course curriculum
Work placements
Aims
Specification of controlled education, way of implementation and compensation for absences
Recommended optional programme components
Prerequisites and corequisites
Basic literature
CAMPBELL, Neil a Jane REECE. Biologie, 1. vyd. Computer press, 2006. ISBN 80-251-1178-4. (CS)
NEČAS, Oldřich et al. Obecná biologie pro lékařské fakulty. 3. přepracované vyd. Jinočany: H+H, 2000. ISBN 80-86022-46-3. (CS)
Recommended reading
Classification of course in study plans
Type of course unit
Lecture
Teacher / Lecturer
Syllabus
2. Cellular memory
3. Transcription
4. Translation
5. Genetic expression control
6. DNA replication
7. Biomembranes and energy transformation
8. Transport of substances
9. Organisation of cellular core
10. Cytoskelet and molecular engines
11. Cellular cycle
12. Cellular signalling
13. Clone and stem cells
Laboratory exercise
Teacher / Lecturer
Syllabus
2. Procaryontic cells: native mounts of fungi , Gram coloring – permanent bacteria mounts.
3. Eucaryontic cells, cytochemical methods for study of cells: Protoyoa, Elodea – preparation of native mounts. Feulgen’s reaction, RNA coloring, Gram’s coloring.
4. Tissue and cellular cultures: the basic principles of cultivation, cryoconservation, collections, applications.
5. Electron microscopy: the principle of the ELM method, methods of preparation of mounts, demonstration –the transmission and scanning electron microscope, the free-etching apparatus, evaluation of ELM images
6. Radioactive isotopes in cellular biology: the principle of utilisation of compounds marked with isotopes, utilisation of radioisotopes for research of specific inhibitors – evaluation of experimental studies, autoradiographical methods – the principle.
7. Impact of deep freezing on cells.Viability tests, detection of membrane integrity disorders (yeast, animal and human cells in vitro), quantitative determination of living and dead cells.
8. Cytotoxicity testing methods – impact of deep freezing on yeast cells, practical applications, in vitro systems, evaluation of the experiment with in vitro testing on human cells.
9. Biomembranes: structure and functions, fusion of cells – an experiment on human and chicken erytrocytes, osmotic effects – an experiment on human erytrocytes, applications (dialysis, fusion of cells – hybridomic technology).
10. Analysis of the cellular cycle: cdc yeast mutants – monitoring of the morphological changes during the cellular cycle, analysis of the growth curve (human cells in vitro, yeast), flow cytometry – the principle, evaluation of histograms.
11. Mitotic division. Coloring of chromosomes, monitoring of permanent mounts – plant meristemes, human lymphocytes.
12. Methods of cellular research (seminar)
13. Internet sources for biomedical research: sources for cellular biology (tissue and line banks, antibodies, catalogs), instruction resources and biomedical information portals, publication databases.