Course detail

Laboratory Technology for Genomics and Proteomics

FEKT-FLABAcad. year: 2011/2012

The course is focused on laboratory equipment used in genomics and proteomics. The study is oriented to knowledge on DNA and proteom's analysis methods.

Language of instruction

Czech

Number of ECTS credits

6

Mode of study

Not applicable.

Guarantor

doc. Ing. Jana Kolářová, Ph.D.

Department

Department of Biomedical Engineering (UBMI)

Learning outcomes of the course unit

Overview of biophysic method for biological data analysis. Knowledge and understanding to laboratory devices used in genomics and proteomics.

Prerequisites

The subject knowledge on the Bachelor's degree level is requested.

Co-requisites

Not applicable.

Planned learning activities and teaching methods

Teaching methods depend on the type of the course unit as specified in the article 7 of BUT Rules for Studies and Examinations.

Assesment methods and criteria linked to learning outcomes

Requirements for completion of a course are specified by a regulation issued by the lecturer responsible for the course and updated for every year.

Course curriculum

Overview of methods used in genomics and proteomics. Basic term definition. Small laboratory devices. DNA sequencing. DNA and protein sample preparation. Flow cytometry. Chromatography. Spectrometry.

Work placements

Not applicable.

Aims

Knowledge of information structure in biophysical methods for biological data analysis. Understanding to used methods and construction of laboratory devices.

Specification of controlled education, way of implementation and compensation for absences

Limmitations of controlled teaching and its procedures are specified by a regulation issued by the lecturer responsible for the course and updated for every year.

Recommended optional programme components

Not applicable.

Prerequisites and corequisites

Not applicable.

Basic literature

Skoog,D.A.-Holler,F.J.-Nieman,T.A.: Principles of Instrumental Analysis, Saunders College Publishing, 1998, 849 s.
Skoog, D.A.-West, D.M.-Holler,F.J.: Fundamentals of Analytical chemistry, 7th edition, Saunders College Publishing, 1996, 659 s. Skoog, D.A.-West, D.M.-Holler,F.J.: Fundamentals of Analytical chemistry, 7th edition, Saunders College Publishing, 1996, 659 s.

Recommended reading

Not applicable.

Classification of course in study plans

  • Programme BTBIO-F Master's

    branch F-BTB , 2. year of study, winter semester, compulsory

  • Programme EEKR-CZV lifelong learning

    branch ET-CZV , 1. year of study, winter semester, compulsory

Type of course unit

 

Lecture

26 hours, optionally

Teacher / Lecturer

doc. Ing. Jana Kolářová, Ph.D.

Syllabus

1. Overview of methods used in genomics and proteomics. Basic term definition.
2. Small laboratory devices.
3. DNA sequencing - sample preparation, sample separation.
4. DNA sequencing - sample preparation, PCR.
5. DNA sequencing.
6. Protein analysis - sample preparation, HPLC.
7. Flow cytometry.
8. Chromatography.
9. Spectrometry - overview of spectrometry methods.
10. Mass spectrometry, absorption spectrometry, fluorescence spectrometry.
11. Natural and non-natural fluorescence biological molecules, fluorescence marks.
12. Fluorescence marks of DNA and proteins.
13. Colorimetry.

Laboratory exercise

26 hours, compulsory

Teacher / Lecturer

doc. Ing. Jana Kolářová, Ph.D.

Syllabus

1. Laboratory introduction and organization of laboratory works.
2. Sample preparation.
3. DNA separation by gel electrophoresis.
4. Quality control of sample separation.
5. Amplification of DNA by PCR.
6. DNA sequencing of plant samples.
7. Sequencing evaluation, sample identification.
8. DNA analysis by spectrophotometer CE2502.
9. DNA analysis by spectrophotometer CE3041.
10. Protein molecules preparing.
11. Protein analysis by spectrophotometer CE2502.
12. Protein analysis by spectrophotometer CE3041.
13. Practical test.

The other activities

13 hours, compulsory

Teacher / Lecturer

doc. Ing. Jana Kolářová, Ph.D.

Syllabus

1. Self-study of principles of laboratory devices, which will be used in practical lessons.
2. DNA samples used in practical lessons.
3. Work on individual projects.