Detail publikačního výsledku

Spectrometric and Voltammetric Analysis of Urease: Nickel Nanoelectrode as an Electrochemical Sensor

HUBÁLEK, J.; HRADECKÝ, J.; ADAM, V.; KRYŠTOFOVÁ, O.; HÚSKA, D.; MASAŘÍK, M.; TRNKOVÁ, L.; HORNA, A.; KLOSOVÁ, K.; ADÁMEK, M.; ZEHNÁLEK, J.; KIZEK, R.

Original Title

Spectrometric and Voltammetric Analysis of Urease: Nickel Nanoelectrode as an Electrochemical Sensor

English Title

Spectrometric and Voltammetric Analysis of Urease: Nickel Nanoelectrode as an Electrochemical Sensor

Type

Peer-reviewed article not indexed in WoS or Scopus

Original Abstract

Urease is the enzyme catalyzing the hydrolysis of urea into carbon dioxide and ammonia. This enzyme is substrate-specific, which means that the enzyme catalyzes the hydrolysis of urea only. This feature is a basic diagnostic criterion used in the determination of many bacteria species. Most of the methods utilized for detection of urease are based on analysis of its enzyme activity (the hydrolysis of urea). The aim of this work was to detect urease indirectly by spectrometric method and directly by voltammetric methods. As spectrometric method we used is called indophenol assay.

English abstract

Urease is the enzyme catalyzing the hydrolysis of urea into carbon dioxide and ammonia. This enzyme is substrate-specific, which means that the enzyme catalyzes the hydrolysis of urea only. This feature is a basic diagnostic criterion used in the determination of many bacteria species. Most of the methods utilized for detection of urease are based on analysis of its enzyme activity (the hydrolysis of urea). The aim of this work was to detect urease indirectly by spectrometric method and directly by voltammetric methods. As spectrometric method we used is called indophenol assay.

Keywords

urease, electrochemical methods, nanotechnology, nanotube, nickel electrode, hanging mercury drop electrode, spectrometry

Key words in English

urease, electrochemical methods, nanotechnology, nanotube, nickel electrode, hanging mercury drop electrode, spectrometry

Authors

HUBÁLEK, J.; HRADECKÝ, J.; ADAM, V.; KRYŠTOFOVÁ, O.; HÚSKA, D.; MASAŘÍK, M.; TRNKOVÁ, L.; HORNA, A.; KLOSOVÁ, K.; ADÁMEK, M.; ZEHNÁLEK, J.; KIZEK, R.

Released

16.07.2007

Publisher

MDPI

ISBN

1424-8220

Periodical

SENSORS

Volume

7

Number

1

State

Swiss Confederation

Pages from

1238

Pages to

1255

Pages count

18

URL

http://www.mdpi.com/1424-8220/7/7/1238

Full text in the Digital Library

http://hdl.handle.net/11012/68386

BibTex

@article{BUT44511,
  author="HUBÁLEK, J. and HRADECKÝ, J. and ADAM, V. and KRYŠTOFOVÁ, O. and HÚSKA, D. and MASAŘÍK, M. and TRNKOVÁ, L. and HORNA, A. and KLOSOVÁ, K. and ADÁMEK, M. and ZEHNÁLEK, J. and KIZEK, R.",
  title="Spectrometric and Voltammetric Analysis of Urease: Nickel Nanoelectrode as an Electrochemical Sensor",
  journal="SENSORS",
  year="2007",
  volume="7",
  number="1",
  pages="1238--1255",
  doi="10.3390/s7071238",
  issn="1424-8220",
  url="http://www.mdpi.com/1424-8220/7/7/1238"
}

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