Publication detail

Use of Calcium Chloride for Removal of Polysaccharides During DNA Isolation from Tropical Fruit Products

FIALOVÁ, L. ŽYLKOVÁ, K. MÁROVÁ, I.

Original Title

Use of Calcium Chloride for Removal of Polysaccharides During DNA Isolation from Tropical Fruit Products

Type

abstract

Language

English

Original Abstract

Food products made out of certain fruits (such as mango) may be subject to adulteration. The adulteration may be detected by various methods, including PCR-based ones. However, PCR-based methods are susceptible to inhibition if the DNA sample is contaminated with certain polysaccharides. Furthermore, polysaccharide contamination is notoriously difficult to remove. In this work we demonstrate the use of calcium chloride for successful removal of potentially inhibitory polysaccharides from samples which are known to contain them (mango and banana puree). We also address the potential inhibitory effect of calcium chloride itself and we show a way in which polysaccharide precipitation by calcium chloride may be added to a DNA isolation protocol without hampering the amplifiability of the isolated DNA.

Keywords

PCR, DNA isolation, polysaccharides, calcium chloride, mango

Authors

FIALOVÁ, L.; ŽYLKOVÁ, K.; MÁROVÁ, I.

Released

23. 10. 2023

Publisher

Institute of Analytical Chemistry of the CAS, v. v. i., Veveri 97, 602 00 Brno, Czech Republic

Location

Brno

ISBN

978-80-908154-0-7

Book

CECE 2023 18th International Interdisciplinary Meeting on Bioanalysis

Edition

1

Edition number

1

Pages from

50

Pages to

50

Pages count

1

URL

https://www.ce-ce.org/user_uploads/CECE_2023/CECE%202023%20Proceedings.pdf

BibTex

@misc{BUT185429,
  author="Lenka {Fialová} and Kateřina {Žylková} and Ivana {Márová}",
  title="Use of Calcium Chloride for Removal of Polysaccharides During DNA Isolation from
Tropical Fruit Products",
  booktitle="CECE 2023 18th International Interdisciplinary Meeting on Bioanalysis",
  year="2023",
  series="1",
  edition="1",
  pages="50--50",
  publisher="Institute of Analytical Chemistry of the CAS, v. v. i., Veveri 97, 602 00 Brno, Czech Republic",
  address="Brno",
  isbn="978-80-908154-0-7",
  url="https://www.ce-ce.org/user_uploads/CECE_2023/CECE%202023%20Proceedings.pdf",
  note="abstract"
}