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Bachelor's Thesis
Author of thesis: Ing. Martin Vengh
Acad. year: 2015/2016
Supervisor: Ing. Lenka Štrbková, Ph.D.
Reviewer: Ing. Aneta Křížová, Ph.D.
Surface treatment of materials for the cell-surface interaction and imitating intrinsic environment of cells is still subject of matter for their potentional usage in the bioengineering. One of the options to modify the surface of material is exhibiting them to effects of plasma of electrical discharge in steam of cyclopropylamine. In this type of plasma, the low pressure plasma polymerization takes place, which creates thin amine-rich layer. There is an assumption that this type of layer is appropriate for cell adhesion and proliferation. As a superior technique for a label-free monitoring of the cell-surface interaction, multimodal holographic microscope (MHM) Q-Phase was exploited to determine the biocompatibility of materials. MHM enables observation in quantitative phase imaging, where the phase is directly proportional to the cell dry mass. This gives opportunity to define various features able to determine the biocompatibility of materials. According to the results amine-rich films enhanced the conditions for the cell adhesion and proliferation.
Multimodal holographic microscope, quantitative phase imaging, plasma polymerization, cell adhesion
Date of defence
22.06.2016
Result of the defence
Defended (thesis was successfully defended)
Grading
D
Language of thesis
Czech
Faculty
Fakulta strojního inženýrství
Department
Institute of Physical Engineering
Study programme
Applied Sciences in Engineering (B3901-3)
Field of study
Physical Engineering and Nanotechnology (B-FIN)
Composition of Committee
prof. RNDr. Tomáš Šikola, CSc. (předseda) prof. RNDr. Miroslav Liška, DrSc. (místopředseda) prof. RNDr. Bohumila Lencová, CSc. (člen) prof. RNDr. Petr Dub, CSc. (člen) prof. RNDr. Radim Chmelík, Ph.D. (člen) prof. RNDr. Jiří Spousta, Ph.D. (člen) prof. Ing. Ivan Křupka, Ph.D. (člen) prof. RNDr. Pavel Zemánek, Ph.D. (člen) RNDr. Antonín Fejfar, CSc. (člen) doc. Ing. Radek Kalousek, Ph.D. (člen)
Supervisor’s reportIng. Lenka Štrbková, Ph.D.
Grade proposed by supervisor: D
Reviewer’s reportIng. Aneta Křížová, Ph.D.
Grade proposed by reviewer: E
Responsibility: Mgr. et Mgr. Hana Odstrčilová