This thesis was focused on studying of asphalt mixture behaviour with Crumb Rubber modified Bitumen at low temperatures. The work is divided into practical and theoretical part. The practical part is focused on manufacturing of test specimens and using of thermal stress restrained specimen test according to ČSN EN 12697-46. The literature review, description of the specimens manufacturing process and analysis of the measured values are introduced in the theoretical part of the work. Three kinds of test specimens were manufactured from one asphalt mixture.