This diploma thesis deals with the issue of the determination of the frost susceptibility of soils in the subgrades of road structures. The theoretical part compares the ways of testing the frost susceptibility in the Czech Republic and other selected countries of the European Union. Furthermore, it also deals with the development of the frost susceptibility index in the Czech Republic. The empirical part focuses on the laboratory testing of the frost susceptibility of the given sample of soil by the direct frost heave method. Moreover, it also deals with the simulation of penetration of frost by the subgrade and the possibility of shortening the length of the freezing during the direct testing the frost susceptibility.