The dissertation deals with requirements, analysis, description and integration of Single Sign-On solution based on SPNEGO/Kerberos protocol. The thesis provides an overview of the Single Sign-On basic principles and concepts and deals with the Kerberos authentication mechanism in more detail. After introducing the fundaments of the Kerberos protocol, its terminology and common implementations, attention is focused on the services and settings of Microsoft Kerberos implementation in Windows 2000/2003 environment. An authentication solution demonstration is performed on J2EE platform using the authentication filter and plug-in. The thesis also includes a brief overview of integrating the Single Sign-On solution into different architectures of corporate information systems and describes the implementation process of this solution. In conclusion, the usability of Kerberos Single Sign-On solution in today's business sector is analysed.