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Master's Thesis
Author of thesis: Bc. Veronika Ševčíková
Acad. year: 2025/2026
Supervisor: prof. Mgr. Martin Vala, Ph.D.
Reviewer: Ing. Matouš Kratochvíl, Ph.D.
This master thesis focuses on the optical properties of luminescent nanoparticles and their potential applications in various fields, specifically in bioimaging. The biocompatibility and toxicity of the studied luminophores have not been examined in this thesis; their suitability for a given application was estimated mainly based on their optical properties. Three different luminophores were studied, two of them are oxazaborinin-based solid state emitters, and one is diphenylamine-diphenylstilbene-based dual-state emitter exhibiting fluorescence in both solution and the solid state. These luminophores were studied in solid state, in tetrahydrofuran (THF) solution, encapsulated in micelles, in the form of nanoprecipitated nanoparticles, and simple core-shell nanoparticles consisting of a nanoprecipitated core coated in tenzide. The measured optical properties included absorption, excitation, and emission spectra, quantum yield, and fluorescence lifetime. For all nanoparticle samples, particle size, polydispersity index, and zeta potential were determined. Photoisomerization induced by short-wavelength radiation was observed in diphenylamine-diphenylstilbene-based luminophore. Furthermore, emission in the red region of the visible spectrum was observed, which had a relatively long lifetime, a property that could be exploited for time-resolved bioimaging. oxazaborinin-based luminophores emitted in the green region of the visible spectrum; their optical properties were found to be insufficient for bioimaging applications. These luminescent materials could be used in the development of OLEDs or in solar cells.
Fluorescence, dual state emmiters, fluorescence nanoparticles, micelles, core-shell nanoparticles, nanoprecipitation
Date of defence
25.05.2026
Result of the defence
Defended (thesis was successfully defended)
Grading
B
Process of defence
Obhajoba proběhla podle následujícího schématu: prezentace student-vyjádření vedoucí/ho-oponentský posudek-reakce na posudek-diskuse s komisí. Studentka přednesla výborný výtah výsledků své diplomové práce, zodpověděla dotazy oponentské i členů komise, reagovala na připomínky k diplomové práci. V diskusi tak studentka prokázala výbornou schopnost orientace v teoretických i praktických základech problematiky diplomové práce. Komise, s uvážením hodnocení v posudku a vyjádření vedoucí/ho, zhodnotila její diplomovou práci celkově jako velmi dobrou. Pekař: Jakým způsobem se dělalo nanosrážení? Jak se měřily nanočástice v roztoku? Jančovičová: Jaké koncentrace tenzidů se používaly a na jakém základě byly vybrány? Lehocký: Jak si vysvětlujete stabilizaci systému? Jaké pH měl roztok při měření nanočástic?
Language of thesis
Czech
Faculty
Fakulta chemická
Department
Institute of Physical and Applied Chemistry
Study programme
Chemistry and Chemical Technologies (NPCP_CHCHTE)
Composition of Committee
doc. Ing. Zdenka Kozáková, Ph.D. (člen) prof. Ing. Miloslav Pekař, CSc. (předseda) doc. Ing. Viera Jančovičová, Ph.D. (člen) prof. Ing. Michal Veselý, CSc. (místopředseda) prof. Ing. Marián Lehocký, Ph.D. (člen) prof. Mgr. Martin Vala, Ph.D. (člen)
Supervisor’s reportprof. Mgr. Martin Vala, Ph.D.
Grade proposed by supervisor: B
Reviewer’s reportIng. Matouš Kratochvíl, Ph.D.
Grade proposed by reviewer: C
Responsibility: Mgr. et Mgr. Hana Odstrčilová