This thesis deals with the preparation and characterization of electrochemical biosensors based on carbon inks modified with carbon nanomaterials. The introductory section presents a literature review focused on the principles, fabrication and application of electrochemical biosensors, with an emphasis on carbon paste elecreodes and the oissubulities of their modification using carbon nanoparticles and other substances.
The thesis also includes practial familiarization with screen-printing and stencil-printing methods for electrode fabrication, which represent a promising technology for the production of low-cost and disposable sensor platforms.
The experimental section involved the preparation of screen-printed and stencil-printed electrodes from unmodified carbon paste, graphene paste, and carbon paste supplemented with carbon nanotubes. The prepared electrodes were subjected to vasic electrochemical characterization using electrochemical impedance spectroscopy, cyclic voltammetry and square-wave voltammetry.
The aquired data were processed and evaluated to compare the electrochemical properties of the individual electrode types. Furthermore, albumin molecules were adsorbed ono the electrode surface as a model receptor systém for an affinity biosensor. The effect od albumin adsorption on the electrochemical response of the electrodes was monitored and analyzed using the selected electrochemical methods.
The results showed that the modification of the carbon paste with carbon nanomaterials affects the electrochemical properties of the electrodes, particularly the chargé transfer at the electrode-solution interface and the size of the electroactive surface area. The adsorption of albumin led to measurable changes in electrochemical parameters, confirming the potential of the prepared electrodes for use in affinity biosensors. The measured results were discussed and compared with available literature data. This work contributes to a better understanding of the impact of carbon nanomaterials on the properties of screen-printed electrodes and their applicability in the field of electrochemical biosensors.