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Bachelor's Thesis
Author of thesis: Kamila Michalská
Acad. year: 2025/2026
Supervisor: Ing. Helena Hudečková, Ph.D.
Reviewer: Ing. Lenka Fialová, Ph.D.
This bachelor thesis focuses on the potential application of medicinal mushrooms in cosmetic products, particularly Ganoderma lucidum (Reishi). Extracts from this mushroom were prepared using different solvents (water, ethanol, combined ethanol-water extraction, and a glycerol-water mixture), while their content of selected bioactive compounds, specifically proteins, phenolic compounds, and flavonoids, was evaluated. In addition, the antioxidant activity of the extracts was determined using the TEAC method, and their antimicrobial activity was tested against selected microorganisms (Escherichia coli, Staphylococcus epidermidis, Candida glabrata). The results showed that the glycerol water extract exhibited the highest content of proteins and phenolic compounds, while the ethanol extract showed the highest antioxidant activity, and the ethanol water extract contained the highest amount of flavonoids. All tested extracts demonstrated antimicrobial activity even at low concentrations, with the ethanol and ethanol-water extracts showing the highest activity against Staphylococcus epidermidis, while the glycerol-water extract achieved very good results particularly against Escherichia coli and Candida glabrata. Based on these findings, the glycerol-water extract was selected for application in a model cosmetic cream due to its favorable composition and skin compatibility. It was demonstrated that the addition of the extract did not negatively affect the physical stability of the emulsion system and maintained its microbiological safety. An orientational evaluation using the Visioscope showed a visible improvement in the appearance of the skin surface after cream application, while the immediate effect was probably mainly caused by the emulsion base itself. The results suggest that extracts from Ganoderma lucidum may represent a promising raw material for cosmetic formulations, particularly in products intended for skin hydration and protection.
Medicinal mushrooms, Reishi, extract, cosmetic product, emulsion, hydration, stability
Date of defence
10.06.2026
Result of the defence
Defended (thesis was successfully defended)
Grading
A
Process of defence
Obhajoba proběhla podle následujícího schématu: prezentace studentky-vyjádření vedoucí/ho-oponentský posudek-reakce na posudek-diskuse s komisí. Studentka přednesla výborný výtah výsledků své bakalářské práce, řádně zodpověděla všechny dotazy oponentské i členů komise, pohotově reagovala na připomínky. V diskusi tak studentka prokázala výbornou schopnost orientace v teoretických i praktických základech problematiky bakalářské práce. Komise zhodnotila její bakalářskou práci celkově jako výbornou. Pekař: Co znamená pojem medicinální houba? Jaká je výtěžnost extrakce? Kolik extraktu se dávalo do krému? Obruča: Existují již komerční produkty s touto houbou? Nemají extrakty specifickou vůni? Kozáková: Neměl etanol sám o sobě antibakteriální účinky? Enev: Jaké látky by měly přejít při extrakci z houby do roztoku? Extrahovaly by se z hub i betaglukany?
Language of thesis
Czech
Faculty
Fakulta chemická
Department
Institute of Physical and Applied Chemistry
Study programme
Chemistry for Medical Application (BPCP_CHMA)
Composition of Committee
prof. Ing. Miloslav Pekař, CSc. (předseda) doc. Ing. Zdenka Kozáková, Ph.D. (člen) prof. Ing. Stanislav Obruča, Ph.D. (místopředseda) doc. Ing. Vojtěch Enev, Ph.D. (člen) doc. Ing. Petr Dzik, Ph.D. (člen)
Supervisor’s reportIng. Helena Hudečková, Ph.D.
Grade proposed by supervisor: A
Reviewer’s reportIng. Lenka Fialová, Ph.D.
Grade proposed by reviewer: A
Responsibility: Mgr. et Mgr. Hana Odstrčilová