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Bachelor's Thesis
Author of thesis: Bc. Juraj Bendík
Acad. year: 2024/2025
Supervisor: Ing. Tomáš Vičar, Ph.D.
Reviewer: Ing. Roman Jakubíček, Ph.D.
Quantitative phase microscopy is a modern tool for non-invasive imaging of biological samples, enabling analysis of their structures without the use of contrast agents. This work focuses on the use of deep learning for phase image reconstruction with an emphasis on phase unwrapping, overcoming the limitations of traditional methods. The thesis includes a description of the principles of quantitative phase imaging, with an emphasis on coherent-controlled holographic microscopy and the process of phase image reconstruction, including solutions to problems associated with phase unwrapping. The fundamentals of deep learning, the architecture of U-Net, and its use in biomedicine are also discussed. The literature review focuses on the analysis of modern and traditional methods of phase image unwrapping. It includes approaches based on unwrapping calculations, noise removal, regression, and unsupervised learning methods, evaluating their effectiveness and suitability for different types of data. The practical part includes the implementation and testing of selected deep learning methods, simulation of a dataset with parameter optimization, and evaluation of results on simulated and experimental data from quantitative phase microscopy. The proposed solution is compared with traditional phase unwrapping methods, and the thesis concludes with a summary of the results achieved and their interpretation.
Quantitative phase microscopy, Phase image reconstruction, Phase unwrapping, Deep learning, U-Net architecture
Date of defence
18.06.2025
Result of the defence
Defended (thesis was successfully defended)
Grading
D
Process of defence
Student prezentoval výsledky své práce a komise byla seznámena s posudky. Ing. Čmiel položil otázku na souvislost CCHM zabaleného a simulovaného zabaleného snímku. Student obhájil bakalářskou práci s výhradami a odpověděl na otázky členů komise a oponenta
Language of thesis
Slovak
Faculty
Fakulta elektrotechniky a komunikačních technologií
Department
Department of Biomedical Engineering
Study programme
Biomedical Technology and Bioinformatics (BPC-BTB)
Composition of Committee
prof. Ing. Martin Černý, Ph.D. (předseda) doc. Ing. Radim Kolář, Ph.D. (místopředseda) Ing. Roman Jakubíček, Ph.D. (člen) Ing. Kateřina Šabatová (člen) Ing. Vratislav Čmiel, Ph.D., MBA (člen) MUDr. Tibor Stračina, Ph.D. (člen)
Supervisor’s reportIng. Tomáš Vičar, Ph.D.
Grade proposed by supervisor: D
Reviewer’s reportIng. Roman Jakubíček, Ph.D.
Grade proposed by reviewer: D
Responsibility: Mgr. et Mgr. Hana Odstrčilová