This diploma thesis is focused on the preparation and characterization of vesicular systems formed by multichain ion pair amphiphiles, so-called IPAs. The initial catanionic vesicles were formed by the single-chain tenside CTAB (cetyltrimethylammonium bromide) and the double-chain DHDP (dihexadecyl phosphate). Several different charged tensides were used for stabilization, and the positive single-chain tenside Septonex was found to be the best stabilizer. Subsequently, the effect of cholesterol at different molar concentrations on stabilization and vesicle properties was observed. The vesicle size and stability were studied using dynamic and electrophoretic light scattering (DLS, ELS), their aggregation behavior by fluorescence spectroscopy using pyrene probe, membrane fluidity using fluorescence anisotropy technique and DPH probe, hydration of the outer part of the membrane using generalized polarization and laurdan probe and the effect of temperature on vesicle stability. DLS and ELS measurements show that vesicles with higher cholesterol content, ideally 30 mol.% or more, are more stable. The outer parts of the membranes of these vesicles also exhibit higher strength at these concentrations, as indicated by generalized polarization measurements. Vesicles with 20 and 25 mol.% show higher membrane hydration. Regarding the membrane microviscosity, at low temperatures the cholesterol content had no significant effect, while at higher temperatures the membranes with lower cholesterol contents, i.e. 20 and 25 mol.%, were more fluid. Regarding the effect of temperature, laboratory and refrigerator temperatures were chosen for the observations, the lower temperature had no effect on long-term stability and it was found that solutions of vesicles prepared in this way could be stored at low temperatures.