The diploma thesis is focused on the testing of asphalt mixtures of asphalt concrete containing reclaimed asphalt and laying of the test section. The theoretical part deals with the use of reclaimed asphalt in hot asphalt mixtures, namely the specifics of rejuvenating agents, aging and asphalt mixture. Further more information on the functional properties of asphalt mixtures and the influence of reclaimed asphalt on them is given. The practical part describes testing of mixtures of ACO 11+ and ACO 11+ with 50% reclaimed asphalt and tests on asphalt binders. In order to compare the proposed mixtures, the low thermal properties of ČSN EN 12697-46 and the stiffness modules according to ČSN EN 12697-26. In the last part of the diploma thesis the results of the measured values were compared with each other in order to determine of asfphalt recovery. From the overall test results, it can be seen that the effect of the rejuvenating agent was positive. However, the desired degree of rejuvenation has not been achieved after production.