The diploma thesis deals with the issue of the availability of the new Main Railway Station in Brno, its transfer to other location, modernization or reconstruction and its connection to the public transport system. It focuses in particular on the public transport in Brno affected by the transfer of the railway station and on regional passenger railway transport in the vicinity of Brno. It describes, evaluates and assesses various variants of the transfer of the new railway station in terms of time savings for passengers traveling through the Main Railway Station and their source or destination of travel is in Brno or in the vicinity of Brno. The most suitable appears variant B - the transfer of the station under Petrov.